PMID- 17647084
OWN - NLM
STAT- MEDLINE
DCOM- 20080821
LR  - 20080422
IS  - 0923-9820 (Print)
IS  - 0923-9820 (Linking)
VI  - 19
IP  - 3
DP  - 2008 Jun
TI  - Evaluation of the microbial diversity in a horizontal-flow anaerobic immobilized 
      biomass reactor treating linear alkylbenzene sulfonate.
PG  - 375-85
AB  - The purpose of this work was to assess the degradation of linear alkylbenzene 
      sulfonate (LAS) in a horizontal-flow anaerobic immobilized biomass (HAIB) 
      reactor. The reactor was filled with polyurethane foam where the sludge from a 
      sanitary sewage treatment was immobilized. The hydraulic detention time (HDT) 
      used in the experiments was of 12 h. The reactor was fed with synthetic substrate 
      (410 mg l(-1) of meat extract, 115 mg l(-1) of starch, 80 mg l(-1) of saccharose, 
      320 mg l(-1) of sodium bicarbonate and 5 ml l(-1) of salt solution) in the 
      following stages of operation: SI-synthetic substrate, SII-synthetic substrate 
      with 7 mg l(-1) of LAS, SIII-synthetic substrate with 14 mg l(-1) of LAS and 
      SIV-synthetic substrate containing yeast extract (substituting meat extract) and 
      14 mg l(-1) of LAS, without starch. At the end of the experiment (313 days) a 
      degradation of approximately 35% of LAS was achieved. The higher the 
      concentration of LAS, the greater the amount of foam for its adsorption. This is 
      necessary because the isotherm of LAS adsorption in the foam is linear for the 
      studied concentrations (2 to 50 mg l(-1)). Microscopic analyses of the biofilm 
      revealed diverse microbial morphologies, while Denaturing Gradient Gel 
      Eletrophoresis (DGGE) profiling showed variations in the population of total 
      bacteria and sulphate-reducing bacteria (SRB). The 16S rRNA gene sequencing and 
      phylogenetic analyses revealed that the members of the order Clostridiales were 
      the major components of the bacterial community in the last reactor operation 
      step.
FAU - Duarte, I C S
AU  - Duarte IC
AD  - Department of Hydraulics and Sanitation, School of Engineering of Sao Carlos, 
      University of Sao Paulo, Brazil. Av. Trabalhador Sao-Carlense, no. 400, Sao 
      Carlos, SP 13566-590, Brazil. iolanda.duarte@gmail.com
FAU - Oliveira, L L
AU  - Oliveira LL
FAU - Saavedra, N K D
AU  - Saavedra NK
FAU - Fantinatti-Garboggini, F
AU  - Fantinatti-Garboggini F
FAU - Oliveira, V M
AU  - Oliveira VM
FAU - Varesche, M B A
AU  - Varesche MB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070724
PL  - Netherlands
TA  - Biodegradation
JT  - Biodegradation
JID - 9100834
RN  - 0 (Alkanesulfonic Acids)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (alkylbenzyl sulfonic acid)
SB  - IM
MH  - Adsorption
MH  - Alkanesulfonic Acids/*metabolism
MH  - Anaerobiosis
MH  - *Biomass
MH  - *Bioreactors
MH  - Methanosarcinales/genetics/*metabolism
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
EDAT- 2007/07/25 09:00
MHDA- 2008/08/22 09:00
CRDT- 2007/07/25 09:00
PHST- 2006/10/19 00:00 [received]
PHST- 2007/07/02 00:00 [accepted]
PHST- 2007/07/25 09:00 [pubmed]
PHST- 2008/08/22 09:00 [medline]
PHST- 2007/07/25 09:00 [entrez]
AID - 10.1007/s10532-007-9143-5 [doi]
PST - ppublish
SO  - Biodegradation. 2008 Jun;19(3):375-85. doi: 10.1007/s10532-007-9143-5. Epub 2007 
      Jul 24.