PMID- 1764919 OWN - NLM STAT- MEDLINE DCOM- 19920220 LR - 20190816 IS - 0305-0491 (Print) IS - 0305-0491 (Linking) VI - 99 IP - 2 DP - 1991 TI - Comparison of rat and human major platelet glycoproteins. PG - 399-403 AB - 1. Using electrophoretic techniques combined with various detection methods we ascribed rat platelet glycoproteins (GPs) related to human GPIb, GPIIb and GPIIIa. 2. Rat GPIIb and GPIIIa crossreacted with rabbit polyclonal antibodies against human GPIIb and GPIIIa. 3. Species differences in glycosylation of GPs were shown using various lectins. 4. Molecular mass of rat major GPs was determined by SDS-PAGE (unreduced, reduced, kDa): GPIb (200, 166/26), GPIIb (140, 120/32) and GPIIIa (96, 106). 5. Isoelectric points of rat GPIIb and GPIIIa are shifted to the alkaline region as compared to human related GPs. FAU - Holada, K AU - Holada K AD - Institute of Hematology and Blood Transfusion, Praha, Czechoslovakia. FAU - Dyr, J E AU - Dyr JE FAU - Suttnar, J AU - Suttnar J FAU - Simak, J AU - Simak J LA - eng PT - Comparative Study PT - Journal Article PL - England TA - Comp Biochem Physiol B JT - Comparative biochemistry and physiology. B, Comparative biochemistry JID - 2984730R RN - 0 (Platelet Membrane Glycoproteins) SB - IM MH - Animals MH - Electrophoresis, Gel, Two-Dimensional MH - Electrophoresis, Polyacrylamide Gel MH - Humans MH - Molecular Weight MH - Platelet Membrane Glycoproteins/*chemistry/isolation & purification MH - Rats MH - Rats, Inbred Strains MH - Species Specificity EDAT- 1991/01/01 00:00 MHDA- 1991/01/01 00:01 CRDT- 1991/01/01 00:00 PHST- 1991/01/01 00:00 [pubmed] PHST- 1991/01/01 00:01 [medline] PHST- 1991/01/01 00:00 [entrez] AID - 10.1016/0305-0491(91)90061-h [doi] PST - ppublish SO - Comp Biochem Physiol B. 1991;99(2):399-403. doi: 10.1016/0305-0491(91)90061-h.