PMID- 17649723
OWN - NLM
STAT- MEDLINE
DCOM- 20090930
LR  - 20131121
IS  - 0253-2727 (Print)
IS  - 0253-2727 (Linking)
VI  - 28
IP  - 1
DP  - 2007 Jan
TI  - [Study of molecular mechanism of doxorubicin enhancement of TRAIL, inducing 
      apoptosis of myeloma cell line KM3].
PG  - 30-2
AB  - OBJECTIVE: To investigate the molecular mechanism of doxorubicin enhancement of 
      tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) inducing 
      apoptotic effect on multiple myeloma cell line KM3. METHODS: Apoptosis was 
      studied independently through flow cytometry analysis and TUNEL staining. The 
      expression of death receptor 5 (DR5) and nuclear factor P65 in nuclear was 
      examined by Western blot. RESULTS: The apoptosis ratio of KM3 cells was 20.88%, 
      40.03%, 57.87%, 60.82% respectively when treated with different concentration of 
      TRAIL (10, 20, 50, 100 ng/ml) combining with doxorubicin. It is markedly higher 
      than the group treated with TRAIL or doxorubicin alone. DR5 expression increased 
      while P65 decreased as the doses of doxorubicin increased when KM3 cells treated 
      with doxorubicin (0.5, 1.0, 2.0 and 4.0 microg/ml) plus 20 ng/ml TRAIL. 
      CONCLUSION: Increasing the expression of DR5 and nuclear transferring of P65 are 
      the important molecular mechanism by which doxorubicin enhances TRAIL-inducing 
      apoptosis of KM3 cells.
FAU - Wang, Hua-fang
AU  - Wang HF
AD  - Institute of Hematology, Union Hospital of Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430022, China.
FAU - Chen, Zhao-hui
AU  - Chen ZH
FAU - Sun, Chun-yan
AU  - Sun CY
FAU - Hu, Yu
AU  - Hu Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
RN  - 0 (Receptors, TNF-Related Apoptosis-Inducing Ligand)
RN  - 0 (TNF-Related Apoptosis-Inducing Ligand)
RN  - 0 (Transcription Factor RelA)
RN  - 80168379AG (Doxorubicin)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Cell Line, Tumor
MH  - Doxorubicin/*pharmacology
MH  - Drug Interactions
MH  - Humans
MH  - Multiple Myeloma/metabolism/*pathology
MH  - Receptors, TNF-Related Apoptosis-Inducing Ligand/genetics/metabolism
MH  - TNF-Related Apoptosis-Inducing Ligand/*pharmacology
MH  - Transcription Factor RelA/genetics/metabolism
EDAT- 2007/07/26 09:00
MHDA- 2009/10/01 06:00
CRDT- 2007/07/26 09:00
PHST- 2007/07/26 09:00 [pubmed]
PHST- 2009/10/01 06:00 [medline]
PHST- 2007/07/26 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2007 Jan;28(1):30-2.