PMID- 17650111
OWN - NLM
STAT- MEDLINE
DCOM- 20071025
LR  - 20220309
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 26
IP  - 2
DP  - 2007 Jul
TI  - Immunolabelling of the 5-HT 3B receptor subunit in the central and peripheral 
      nervous systems in rodents.
PG  - 355-66
AB  - The 5-HT(3) receptor is a member of the superfamily of neurotransmitter-gated ion 
      channels involved in fast synaptic signalling and in modulation of 
      neurotransmitter release. As for many other channel receptors, the 
      electrophysiological properties and the functions of the 5-HT(3) receptor are 
      determined by subunit composition of the pentameric channel. Because in situ 
      hybridization did not allow the detection of mRNA encoding the 5-HT(3B) subunit 
      in the rodent central nervous system, or in nearly half of the neurons expressing 
      the 5-HT(3A) subunit in peripheral ganglia, it has been suggested that subunit 
      composition could define at least two 5-HT(3) receptor-expressing neuronal 
      populations. In order to challenge this hypothesis, we have developed polyclonal 
      antibodies directed against a portion of the second intracytoplasmic loop of the 
      mouse 5-HT(3B) subunit. Immunohistochemical analysis in the mouse and the rat 
      revealed that immunolabelling was most prominent in peripheral ganglia, 
      particularly in trigeminal ganglia (TG). In rats, transection or ligature of the 
      infraorbital nerve resulted in a pronounced accumulation of immunoreactive 
      material at the proximal side of the lesioned nerve, and an up-regulation of both 
      subunits in 5-HT(3) receptor-expressing TG neurons. Surprisingly, nearly 100% of 
      neurons expressing 5-HT(3A) subunits were also labelled by anti-5-HT(3B) 
      antibodies. We also detected 5-HT(3B) immunoreactivity in the rat hippocampal CA1 
      layer and in scattered cortical neurons, indicating that detection of 5-HT(3) 
      subunit mRNA by in situ hybridization might not provide really complete mapping 
      of heteromeric 5-HT(3A/B) vs. homomeric 5-HT(3A) receptors in the peripheral and 
      central nervous systems in rodents.
FAU - Doucet, Edith
AU  - Doucet E
AD  - INSERM U677, F-75013 Paris, France.
FAU - Latremoliere, Alban
AU  - Latremoliere A
FAU - Darmon, Michele
AU  - Darmon M
FAU - Hamon, Michel
AU  - Hamon M
FAU - Emerit, Michel B
AU  - Emerit MB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Receptors, Serotonin, 5-HT3)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-ret)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - COS Cells
MH  - Central Nervous System/injuries/*physiology
MH  - Chlorocebus aethiops/physiology
MH  - Constriction, Pathologic/pathology
MH  - DNA/genetics
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Ganglia/drug effects/physiology
MH  - Immunohistochemistry
MH  - Male
MH  - Mice
MH  - Peripheral Nervous System/injuries/*physiology
MH  - Proto-Oncogene Proteins c-ret/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Serotonin, 5-HT3/*physiology
MH  - Up-Regulation/genetics/physiology
EDAT- 2007/07/26 09:00
MHDA- 2007/10/27 09:00
CRDT- 2007/07/26 09:00
PHST- 2007/07/26 09:00 [pubmed]
PHST- 2007/10/27 09:00 [medline]
PHST- 2007/07/26 09:00 [entrez]
AID - EJN5659 [pii]
AID - 10.1111/j.1460-9568.2007.05659.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2007 Jul;26(2):355-66. doi: 10.1111/j.1460-9568.2007.05659.x.