PMID- 17650225
OWN - NLM
STAT- MEDLINE
DCOM- 20071016
LR  - 20080604
IS  - 1751-2972 (Print)
IS  - 1751-2972 (Linking)
VI  - 8
IP  - 3
DP  - 2007 Aug
TI  - HUITAI rapid urease test: a new ultra-rapid biopsy urease test for the diagnosis 
      of Helicobacter pylori infection.
PG  - 139-42
AB  - BACKGROUND: The gastric biopsy urease test is an accurate and robust diagnostic 
      test for Helicobacter pylori infection. Large endoscopy units use their own 
      homemade unbuffered ultra-rapid urease test for diagnosis of H. pylori infection 
      but several commercial rapid urease tests are available. OBJECTIVE: To compare 
      the accuracy and reaction time of a new biopsy urease test, HUITAI rapid urease 
      test, in the diagnosis of H. pylori infection. METHODS: Consecutive patients 
      presenting with dyspepsia to the endoscopy unit, University of Malaya Medical 
      Center were recruited for the study. Patients who were previously treated for H. 
      pylori infection or who had received antibiotics, proton pump inhibitors or 
      bismuth compounds in the preceding 4 weeks were excluded. The H. pylori diagnosis 
      was made based on the homemade rapid urease test, histology and culture of 
      gastric biopsies. Biopsies from the antrum and corpus of the stomach were taken 
      for this purpose. In addition, two antral and corpus biopsies were taken for the 
      HUITAI rapid urease test. A positive diagnosis of H. pylori infection was made 
      when the culture was positive or if both histology and the rapid urease test were 
      positive. A negative diagnosis was made when all tests were negative. The 
      positive reaction time of the HUITAI rapid urease test was carefully timed up to 
      60 min. RESULTS: Two hundred and six patients were recruited in the study. One 
      hundred and twelve were diagnosed as having an H. pylori infection while the 
      other 94 patients were regarded as negative. There were no spoiled tests and no 
      indeterminate results. The sensitivity of the HUITAI rapid urease test was 98.2% 
      (95% confidence interval (CI): 93.7%, 99.8%), specificity, 99.0% (95% CI: 94.2%, 
      100%), positive predictive value, 99.0% (95% CI: 95.1%, 100%), negative 
      predictive value, 97.9% (95% CI: 92.6%, 99.7%). The overall diagnostic accuracy 
      for the HUITAI rapid urease test was 98.5% (95% CI: 96.6, 99.9). The median 
      positive reaction time was 1.0 min (25-75% inter-quartile range [IQR]: 1.0-3.0 
      min). CONCLUSIONS: The HUITAI rapid urease test is highly accurate for the 
      diagnosis of an H. pylori infection and showed a very rapid positive reaction 
      time.
FAU - Goh, Khean-Lee
AU  - Goh KL
AD  - Division of Gastroenterology, Department of Medicine, Faculty of Medicine, 
      University of Malaya, Kuala Lumpur, Malaysia. klgoh56@tm.net.my
FAU - Cheah, Phaik-Leng
AU  - Cheah PL
FAU - Navaratnam, Parasakthi
AU  - Navaratnam P
FAU - Chin, Sow-Chan
AU  - Chin SC
FAU - Xiao, Shu-Dong
AU  - Xiao SD
LA  - eng
PT  - Clinical Trial
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - J Dig Dis
JT  - Journal of digestive diseases
JID - 101302699
RN  - 0 (Reagent Kits, Diagnostic)
RN  - EC 3.5.1.5 (Urease)
SB  - IM
MH  - Adult
MH  - Biopsy
MH  - Female
MH  - Helicobacter Infections/*diagnosis/*enzymology
MH  - Helicobacter pylori/enzymology/metabolism
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Predictive Value of Tests
MH  - Pyloric Antrum/*microbiology/*pathology
MH  - Reagent Kits, Diagnostic/microbiology
MH  - Urease/*analysis/metabolism
EDAT- 2007/07/26 09:00
MHDA- 2007/10/17 09:00
CRDT- 2007/07/26 09:00
PHST- 2007/07/26 09:00 [pubmed]
PHST- 2007/10/17 09:00 [medline]
PHST- 2007/07/26 09:00 [entrez]
AID - CDD300 [pii]
AID - 10.1111/j.1443-9573.2007.00300.x [doi]
PST - ppublish
SO  - J Dig Dis. 2007 Aug;8(3):139-42. doi: 10.1111/j.1443-9573.2007.00300.x.