PMID- 17651977
OWN - NLM
STAT- MEDLINE
DCOM- 20071031
LR  - 20220409
IS  - 0969-9961 (Print)
IS  - 0969-9961 (Linking)
VI  - 27
IP  - 3
DP  - 2007 Sep
TI  - Enhancement of neuroplasticity through upregulation of beta1-integrin in human 
      umbilical cord-derived stromal cell implanted stroke model.
PG  - 339-53
AB  - Neuroplasticity subsequent to functional angiogenesis is an important goal for 
      cell-based therapy of ischemic neural tissues. At present, the cellular and 
      molecular mechanisms involved are still not well understood. In this study, we 
      isolated mesenchymal stem cells (MSCs) from Wharton's jelly (WJ) to obtain 
      clonally expanded human umbilical cord-derived mesenchymal stem cells (HUCMSCs) 
      with multilineage differentiation potential. Experimental rats receiving 
      intracerebral HUCMSC transplantation showed significantly improved neurological 
      function compared to vehicle-treated control rats. Cortical neuronal activity, as 
      evaluated by proton MR spectroscopy (1H-MRS), also increased considerably in the 
      transplantation group. Transplanted HUCMSCs migrated towards the ischemic 
      boundary zone and differentiated into glial, neuronal, doublecortin+, CXCR4+, and 
      vascular endothelial cells to enhance neuroplasticity in the ischemic brain. In 
      addition, HUCMSC transplantation promoted the formation of new vessels to 
      increase local cortical blood flow in the ischemic hemisphere. Modulation by stem 
      cell-derived macrophage/microglial interactions, and increased beta1-integrin 
      expression, might enhance this angiogenic architecture within the ischemic brain. 
      Inhibition of beta1-integrin expression blocked local angiogenesis and reduced 
      recovery from neurological deficit. In addition, significantly increased 
      modulation of neurotrophic factor expression was also found in the HUCMSC 
      transplantation group. In summary, regulation of beta1-integrin expression plays 
      a critical role in the plasticity of the ischemic brain after the implantation of 
      HUCMSCs.
FAU - Ding, Dah-Ching
AU  - Ding DC
AD  - Graduate Institute of Medical Science, School of Medicine, Buddhist Tzu Chi 
      General Hospital, Tzu-Chi University, Department of Obstetrics and Gynecology, 
      Hualien, Taiwan 970.
FAU - Shyu, Woei-Cherng
AU  - Shyu WC
FAU - Chiang, Ming-Fu
AU  - Chiang MF
FAU - Lin, Shinn-Zong
AU  - Lin SZ
FAU - Chang, Ying-Chen
AU  - Chang YC
FAU - Wang, Hsiao-Jung
AU  - Wang HJ
FAU - Su, Ching-Yuan
AU  - Su CY
FAU - Li, Hung
AU  - Li H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070618
PL  - United States
TA  - Neurobiol Dis
JT  - Neurobiology of disease
JID - 9500169
RN  - 0 (Dcx protein, rat)
RN  - 0 (Doublecortin Protein)
RN  - 0 (Integrin beta1)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Brain/blood supply
MH  - Brain Ischemia/*therapy
MH  - Cell Differentiation/physiology
MH  - Cell Movement/physiology
MH  - Doublecortin Protein
MH  - Humans
MH  - Immunohistochemistry
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Integrin beta1/*biosynthesis
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/cytology
MH  - Microscopy, Confocal
MH  - *Neovascularization, Physiologic
MH  - Neuronal Plasticity/*physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Recovery of Function
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stroke/*therapy
MH  - Stromal Cells/transplantation
MH  - Umbilical Cord/cytology
MH  - Up-Regulation
EDAT- 2007/07/27 09:00
MHDA- 2007/11/01 09:00
CRDT- 2007/07/27 09:00
PHST- 2007/03/07 00:00 [received]
PHST- 2007/05/03 00:00 [revised]
PHST- 2007/06/04 00:00 [accepted]
PHST- 2007/07/27 09:00 [pubmed]
PHST- 2007/11/01 09:00 [medline]
PHST- 2007/07/27 09:00 [entrez]
AID - S0969-9961(07)00114-3 [pii]
AID - 10.1016/j.nbd.2007.06.010 [doi]
PST - ppublish
SO  - Neurobiol Dis. 2007 Sep;27(3):339-53. doi: 10.1016/j.nbd.2007.06.010. Epub 2007 
      Jun 18.