PMID- 17652412
OWN - NLM
STAT- MEDLINE
DCOM- 20080108
LR  - 20131121
IS  - 0022-3077 (Print)
IS  - 0022-3077 (Linking)
VI  - 98
IP  - 4
DP  - 2007 Oct
TI  - Neurotrophic factors promote and enhance locomotor recovery in untrained 
      spinalized cats.
PG  - 1988-96
AB  - In spinal cats, locomotor recovery without rehabilitation is limited, but 
      weight-bearing stepping returns with treadmill training. We studied whether 
      neurotrophins administered to the injury site also restores locomotion in 
      untrained spinal cats and whether combining both neurotrophins and training 
      further improves recovery. Ordinary rat fibroblasts or a mixture of fibroblasts 
      secreting brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) 
      (Fb-NTF) were grafted into T12 spinal transection sites. Cats with each type of 
      transplant were divided into two groups: one receiving daily training and the 
      other receiving no training. As expected, trained cats with/without 
      neurotrophin-producing transplants could step on the treadmill. Untrained cats 
      without neurotrophin-producing transplants could not locomote. However, untrained 
      cats with neurotrophin-secreting transplants performed plantar weight-bearing 
      stepping at speeds up to 0.8 m/s as early as 2 wk after transection. Locomotor 
      capability and stance lengths in these animals were similar to those in animals 
      receiving training alone, suggesting that administration of BDNF/NT-3 was 
      equivalent to treadmill training in restoring locomotion in chronically 
      spinalized cats. Cats receiving both interventions showed the greatest 
      improvement in step length. Anatomical evaluation indicated that all transections 
      were complete and that axons did not enter the cord caudal to the graft. Thus 
      BDNF/NT-3 secreting fibroblasts were equivalent to training in their ability to 
      engage the locomotor circuitry in chronic spinal cats. Furthermore, the rapid 
      time-course of recovery and the absence of axonal growth through the transplants 
      indicate that the restorative mechanisms were not related to supraspinal axonal 
      growth. Finally, the results show that transplants beneficial in rodents are 
      applicable to larger mammals.
FAU - Boyce, Vanessa S
AU  - Boyce VS
AD  - Department of Neurobiology and Anatomy, Drexel University College of Medicine, 
      Philadelphia, PA 19129, USA.
FAU - Tumolo, Maureen
AU  - Tumolo M
FAU - Fischer, Itzhak
AU  - Fischer I
FAU - Murray, Marion
AU  - Murray M
FAU - Lemay, Michel A
AU  - Lemay MA
LA  - eng
GR  - NS24707/NS/NINDS NIH HHS/United States
GR  - NS41975/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20070725
PL  - United States
TA  - J Neurophysiol
JT  - Journal of neurophysiology
JID - 0375404
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neurotrophin 3)
SB  - IM
CIN - J Neurophysiol. 2007 Oct;98(4):1845-6. PMID: 17686909
MH  - Animals
MH  - Biomechanical Phenomena
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Cats
MH  - Data Interpretation, Statistical
MH  - Decerebrate State/*drug therapy/*physiopathology/therapy
MH  - Exercise Therapy
MH  - Female
MH  - Fibroblasts/drug effects/transplantation
MH  - Functional Laterality/physiology
MH  - Hindlimb/physiology
MH  - Immunohistochemistry
MH  - Locomotion/*physiology
MH  - Nerve Growth Factors/*pharmacology
MH  - Neuronal Plasticity/drug effects/physiology
MH  - Neurotrophin 3/pharmacology
MH  - Spinal Cord/cytology
EDAT- 2007/07/27 09:00
MHDA- 2008/01/09 09:00
CRDT- 2007/07/27 09:00
PHST- 2007/07/27 09:00 [pubmed]
PHST- 2008/01/09 09:00 [medline]
PHST- 2007/07/27 09:00 [entrez]
AID - 00391.2007 [pii]
AID - 10.1152/jn.00391.2007 [doi]
PST - ppublish
SO  - J Neurophysiol. 2007 Oct;98(4):1988-96. doi: 10.1152/jn.00391.2007. Epub 2007 Jul 
      25.