PMID- 17652637
OWN - NLM
STAT- MEDLINE
DCOM- 20071203
LR  - 20240414
IS  - 1525-1578 (Print)
IS  - 1525-1578 (Linking)
VI  - 9
IP  - 4
DP  - 2007 Sep
TI  - ''Minor'' BCL2 breakpoints in follicular lymphoma: frequency and correlation with 
      grade and disease presentation in 236 cases.
PG  - 530-7
AB  - Follicular lymphomas are frequently associated with the t(14;18)(q32;q21). This 
      translocation can be detected by karyotype, polymerase chain reaction (PCR), and 
      fluorescence in situ hybridization (FISH). In addition to the breakpoints 
      currently used for diagnosis located in the major breakpoint region (MBR) and the 
      minor cluster region (mcr), recent studies have reported the existence of other 
      breakpoints (3' BCL2, 5'mcr, and icr). In this study, we examined the frequency 
      of all five breakpoints in 236 cases of follicular lymphomas by real-time PCR 
      analysis. The distribution of breakpoint sites consisted of MBR in 118 cases 
      (50%), mcr in 11 (5%), icr in 32 (13%), 3' BCL2 in 13 (6%), and 5' mcr in three 
      cases (1%). These findings illustrate significantly higher frequency of the icr 
      breakpoint as compared with the more frequently studied mcr. Correlation of 
      breakpoints with histology showed that MBR breakpoints occur more frequently in 
      grade 2 lymphomas (P = 0.042). A majority of the PCR-negative cases (75%) 
      contained an IGH/BCL2 translocation with FISH methods, suggesting the presence of 
      other BCL2 breakpoints. Correlation of breakpoints with survival did not reveal 
      significant differences. Diagnostic laboratories should consider expanding their 
      PCR methods to include other BCL2 breakpoints and correlating with FISH methods 
      when appropriate.
FAU - Weinberg, Olga K
AU  - Weinberg OK
AD  - Stanford University, Department of Pathology, 300 Pasteur Dr., Room L235, 
      Stanford, CA 94305, USA. okw@stanford.edu
FAU - Ai, Weiyun Z
AU  - Ai WZ
FAU - Mariappan, M Rajan
AU  - Mariappan MR
FAU - Shum, Carol
AU  - Shum C
FAU - Levy, Ronald
AU  - Levy R
FAU - Arber, Daniel A
AU  - Arber DA
LA  - eng
GR  - P01 CA034233/CA/NCI NIH HHS/United States
GR  - R37 CA033399/CA/NCI NIH HHS/United States
GR  - CA 33399/CA/NCI NIH HHS/United States
GR  - CA 34233/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20070725
PL  - United States
TA  - J Mol Diagn
JT  - The Journal of molecular diagnostics : JMD
JID - 100893612
RN  - 0 (BCL6 protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Proto-Oncogene Proteins c-bcl-6)
SB  - IM
MH  - *Chromosome Breakage
MH  - DNA-Binding Proteins/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, Follicular/diagnosis/*genetics/*pathology/therapy
MH  - Polymerase Chain Reaction
MH  - Prognosis
MH  - Proto-Oncogene Proteins c-bcl-2/*genetics
MH  - Proto-Oncogene Proteins c-bcl-6
MH  - Survival Analysis
MH  - Translocation, Genetic
PMC - PMC1975105
EDAT- 2007/07/27 09:00
MHDA- 2007/12/06 09:00
PMCR- 2008/09/01
CRDT- 2007/07/27 09:00
PHST- 2007/07/27 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/07/27 09:00 [entrez]
PHST- 2008/09/01 00:00 [pmc-release]
AID - S1525-1578(10)60425-4 [pii]
AID - 07-0038 [pii]
AID - 10.2353/jmoldx.2007.070038 [doi]
PST - ppublish
SO  - J Mol Diagn. 2007 Sep;9(4):530-7. doi: 10.2353/jmoldx.2007.070038. Epub 2007 Jul 
      25.