PMID- 17655880
OWN - NLM
STAT- MEDLINE
DCOM- 20071003
LR  - 20131121
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 81
IP  - 7
DP  - 2007 Jul 26
TI  - D-Psicose inhibits the expression of MCP-1 induced by high-glucose stimulation in 
      HUVECs.
PG  - 592-9
AB  - Monocyte chemoattractant protein-1 (MCP-1) is a 76-amino-acid chemokine thought 
      to be the major chemotactic factor for monocytes. MCP-1 is found in 
      macrophage-rich areas of atherosclerotic lesions. Recent report indicates that 
      MCP-1 is induced by glucose-stimulation, raising the important link between 
      diabetes mellitus and atherosclerosis. One of the rare sugars, d-psicose 
      (d-ribo-2-hexulose) is present in small quantities in commercial carbohydrate 
      complexes, however the physiological functions of d-psicose have not been 
      evaluated. In this study, we examined the effects of d-psicose on MCP-1 
      expression in human umbilical vein endothelial cells (HUVECs). Results showed 
      that MCP-1 mRNA and protein were stimulated following exposure to 22.4 mM 
      glucose. Transcriptional activity of MCP-1 promoter paralleled endogenous 
      expression of the gene and this activity was dependent on the dose of d-glucose. 
      d-Psicose inhibited these effects. Next we used inhibitors of selected signal 
      transduction pathways to show that high-glucose (HG) stimulated MCP-1 promoter 
      activity was sensitive to p38-Mitogen-Activated Protein Kinase (p38-MAPK) pathway 
      inhibitor. As expected, a dominant-negative p38-MAPK abolished the stimulatory 
      effect of HG on the promoter activity. To incubate the cells with HG and 
      d-psicose reduced the activation of p38-MAPK. Together, these results indicate 
      that the d-psicose suppression of HG induced MCP-1 expression is mediated in part 
      by inhibition of the p38-MAPK pathway and raise the possibility that d-psicose 
      may be of therapeutic value in the treatment of diseases such as atherosclerosis.
FAU - Murao, Koji
AU  - Murao K
AD  - Division of Endocrinology and Metabolism, Department of Internal Medicine, 
      Faculty of Medicine, Kagawa University, Kagawa, Japan. mkoji@kms.ac.jp
FAU - Yu, Xiao
AU  - Yu X
FAU - Cao, Wen M
AU  - Cao WM
FAU - Imachi, Hitomi
AU  - Imachi H
FAU - Chen, Ke
AU  - Chen K
FAU - Muraoka, Tomie
AU  - Muraoka T
FAU - Kitanaka, Noriko
AU  - Kitanaka N
FAU - Li, Junhun
AU  - Li J
FAU - Ahmed, Rania A M
AU  - Ahmed RA
FAU - Matsumoto, Kensuke
AU  - Matsumoto K
FAU - Nishiuchi, Takamasa
AU  - Nishiuchi T
FAU - Tokuda, Masaaki
AU  - Tokuda M
FAU - Ishida, Toshihiko
AU  - Ishida T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070703
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 23140-52-5 (psicose)
RN  - 30237-26-4 (Fructose)
RN  - EC 1.13.12.- (Luciferases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/*antagonists & inhibitors/biosynthesis/genetics
MH  - Endothelial Cells/*drug effects
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fructose/*pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Genes, Reporter/genetics
MH  - Glucose/*pharmacology
MH  - Humans
MH  - Immunohistochemistry
MH  - Luciferases/biosynthesis/genetics
MH  - Phosphorylation
MH  - Plasmids/genetics
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction/drug effects
MH  - Stimulation, Chemical
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
EDAT- 2007/07/28 09:00
MHDA- 2007/10/04 09:00
CRDT- 2007/07/28 09:00
PHST- 2007/02/13 00:00 [received]
PHST- 2007/06/06 00:00 [revised]
PHST- 2007/06/24 00:00 [accepted]
PHST- 2007/07/28 09:00 [pubmed]
PHST- 2007/10/04 09:00 [medline]
PHST- 2007/07/28 09:00 [entrez]
AID - S0024-3205(07)00483-3 [pii]
AID - 10.1016/j.lfs.2007.06.019 [doi]
PST - ppublish
SO  - Life Sci. 2007 Jul 26;81(7):592-9. doi: 10.1016/j.lfs.2007.06.019. Epub 2007 Jul 
      3.