PMID- 17656256
OWN - NLM
STAT- MEDLINE
DCOM- 20080624
LR  - 20181113
IS  - 1873-4456 (Electronic)
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 176
IP  - 2
DP  - 2007 Jul 15
TI  - Cytogenetic and molecular study of the PRDX4 gene in a t(X;18)(p22;q23): a 
      cautionary tale.
PG  - 131-6
AB  - The PRDX4 gene located at Xp22 encodes for a member of the peroxiredoxin gene 
      family. Genes within this family exhibit thioredoxin-dependent peroxidase 
      activity and have been implicated in cellular functioning, including 
      proliferation and differentiation. Recently, PRDX4 has been identified as a 
      partner gene in a t(X;21) translocation in a patient with acute myeloid leukemia. 
      To determine whether PRDX4 was involved in other translocations, leukemia cells 
      from 15 patients with Xp22 abnormalities were screened for involvement of the 
      gene using fluorescence in situ hybridization (FISH). One sample from a 
      41-year-old woman with acute lymphoblastic leukemia showed three signals when 
      hybridized with the PRDX4 probe. Cytogenetic analysis of the sample had 
      identified a t(X;18)(p22;q23). Assuming that the three signals indicated a break 
      within the PRDX4 gene, we performed FISH experiments and successfully narrowed 
      the breakpoint on chromosome 18 to a 50-kb region. Subsequent analysis using 
      spectral karyotyping showed that the leukemic cells had undergone multiple 
      rearrangements and that a third X chromosome was present, albeit rearranged. 
      Additional FISH experiments revealed that the third PRDX4 signal was the result 
      of a third copy of the gene. Analysis of the other rearrangements has helped to 
      characterize the multiple abnormalities within the leukemic cells. The findings 
      underscore the importance of using multiple techniques when analyzing complex 
      chromosomal rearrangements in malignant cells.
FAU - Gerr, Heidrun D
AU  - Gerr HD
AD  - Institute for Cell and Molecular Pathology, Medizinische Hochschule Hannover, 
      Hannover Germany.
FAU - Nassin, Michele L
AU  - Nassin ML
FAU - Davis, Elizabeth M
AU  - Davis EM
FAU - Jayathilaka, Nimanthi
AU  - Jayathilaka N
FAU - Neilly, Mary E
AU  - Neilly ME
FAU - Schlegelberger, Brigitte
AU  - Schlegelberger B
FAU - Zhang, Yanming
AU  - Zhang Y
FAU - Rowley, Janet D
AU  - Rowley JD
LA  - eng
GR  - R01 CA084405/CA/NCI NIH HHS/United States
GR  - R01 CA084405-05/CA/NCI NIH HHS/United States
GR  - CA84405/CA/NCI NIH HHS/United States
GR  - Howard Hughes Medical Institute/United States
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Core Binding Factor Alpha 2 Subunit)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (RUNX1 protein, human)
RN  - EC 1.11.1.15 (PRDX4 protein, human)
RN  - EC 1.11.1.15 (Peroxiredoxins)
SB  - IM
MH  - Adult
MH  - *Chromosomes, Human, Pair 18
MH  - *Chromosomes, Human, X
MH  - Core Binding Factor Alpha 2 Subunit/genetics
MH  - Cytogenetic Analysis/*methods
MH  - Female
MH  - Gene Duplication
MH  - Humans
MH  - Oncogene Proteins, Fusion/genetics
MH  - Peroxiredoxins/*genetics
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/diagnosis/genetics
MH  - *Translocation, Genetic
PMC - PMC2083648
MID - NIHMS28393
EDAT- 2007/07/28 09:00
MHDA- 2008/06/25 09:00
PMCR- 2008/07/15
CRDT- 2007/07/28 09:00
PHST- 2006/11/30 00:00 [received]
PHST- 2007/02/23 00:00 [revised]
PHST- 2007/03/29 00:00 [accepted]
PHST- 2007/07/28 09:00 [pubmed]
PHST- 2008/06/25 09:00 [medline]
PHST- 2007/07/28 09:00 [entrez]
PHST- 2008/07/15 00:00 [pmc-release]
AID - S0165-4608(07)00209-9 [pii]
AID - 10.1016/j.cancergencyto.2007.03.010 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2007 Jul 15;176(2):131-6. doi: 
      10.1016/j.cancergencyto.2007.03.010.