PMID- 17657088
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20070727
IS  - 1319-2442 (Print)
IS  - 1319-2442 (Linking)
VI  - 14
IP  - 1
DP  - 2003 Jan-Mar
TI  - Comparison of HLA Class I and II Molecular and Serological Typing within Clinical 
      Laboratory.
PG  - 39-42
AB  - In order to develop an adequate tissue typing strategy, we performed human 
      leukocyte antigen (HLA)-A, B and DR generic typing on 235 (120 HLA-A, B and 115 
      HLA-DR) routine clinical samples by polymerase -chain reaction (PCR)-SSP in 
      parallel with conventional serological typing. At the A locus, there were two 
      (1.7%) discrepancies between molecular and serological typing besides 25 (20.8%) 
      serological blanks, which was defined by molecular typing. At the B locus, there 
      were two (1.7%) discrepancies and 30 serological blanks, which were defined by 
      molecular typing. At the DR locus there were two (1.8%) discrepancies and 44 
      serological blanks, which were defined by molecular typing. We conclude that 
      molecular typing is of substantial benefit in the resolution of poorly defined 
      serological antigens. In view of the low percentage of discrepancy between the 
      serological and molecular typing besides the high cost of molecular typing, our 
      policy is to perform HLA typing first by the serological method and to use 
      PCR-SSP as an adjuvant tool.
FAU - Ayed, Khaled
AU  - Ayed K
AD  - Hospital Charles Nicolle - Immunology, Internal Medicine, Tunis, Tunisia.
FAU - Jendoubi, S Ayed
AU  - Jendoubi SA
FAU - Makhlouf, M
AU  - Makhlouf M
FAU - Sfar, I
AU  - Sfar I
FAU - Abdallah, T Ben
AU  - Abdallah TB
LA  - eng
PT  - Journal Article
PL  - Saudi Arabia
TA  - Saudi J Kidney Dis Transpl
JT  - Saudi journal of kidney diseases and transplantation : an official publication of 
      the Saudi Center for Organ Transplantation, Saudi Arabia
JID - 9436968
EDAT- 2007/07/28 09:00
MHDA- 2007/07/28 09:01
CRDT- 2007/07/28 09:00
PHST- 2007/07/28 09:00 [pubmed]
PHST- 2007/07/28 09:01 [medline]
PHST- 2007/07/28 09:00 [entrez]
PST - ppublish
SO  - Saudi J Kidney Dis Transpl. 2003 Jan-Mar;14(1):39-42.