PMID- 17657352
OWN - NLM
STAT- MEDLINE
DCOM- 20080110
LR  - 20190608
IS  - 0716-9760 (Print)
IS  - 0716-9760 (Linking)
VI  - 40
IP  - 1
DP  - 2007
TI  - Multicolor fluorescence in situ hybridization with combinatorial labeling probes 
      enables a detailed karyotype analysis of Larix principis-rupprechtii.
PG  - 23-8
AB  - The chromosomes (2n = 2x = 24) of Larix principis-rupprechtii are composed of six 
      pairs of large metacentrics and six pairs of medium-sized submetacentrics. The 
      identification of homologous pairs is hampered by their high degree of similarity 
      at the morphological level in each group. As one of the most extensively used 
      methods in molecular cytogenetics producing chromosome landmarks, fluorescence in 
      situ hybridization (FISH) has significantly facilitated karyotype construction, 
      especially in species with morphologically similar chromosomes. This study 
      developed a simple but effective use of combinatorial labeling probes to 
      distinguish chromosomes of Larix principis-rupprechtii by multicolor FISH. Three 
      highly repetitive sequences in Larix were selected: 25S rDNA hybridized at all of 
      the secondary constrictions of two pairs of metacentrics and the largest pair of 
      submetacentrics; 5S rDNA hybridized at subtelomeric sites of one pair of 
      metacentrics that also harboured 25S rDNA on different arms; LPD family sequences 
      are tandem repeats hybridized at proximal regions of 22 chromosomes. The three 
      different probes were labeled with only two different labels, hybridized to 
      metaphase chromosomes of Larix principis-rupprechtii, simultaneously visualized, 
      and unequivocally distinguished in a single FISH experiment. These multicolor 
      FISH marks largely improved the karyotype analysis of Larix 
      principis-rupprechtii.
FAU - Liu, Bo
AU  - Liu B
AD  - Laboratory of Cell and Molecular Biology, College of Life Sciences, Tianjin, 
      300071, China.
FAU - Liwang, Qi
AU  - Liwang Q
FAU - Ruiyang, Chen
AU  - Ruiyang C
FAU - Song, Wenqin
AU  - Song W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070719
PL  - England
TA  - Biol Res
JT  - Biological research
JID - 9308271
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Plant)
RN  - 0 (RNA, Ribosomal, 5S)
SB  - IM
MH  - *Chromosome Mapping
MH  - Chromosomes, Plant/genetics
MH  - DNA, Ribosomal/genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Larix/*genetics/physiology
MH  - RNA, Plant/genetics
MH  - RNA, Ribosomal, 5S/genetics
EDAT- 2007/07/28 09:00
MHDA- 2008/01/11 09:00
CRDT- 2007/07/28 09:00
PHST- 2007/07/28 09:00 [pubmed]
PHST- 2008/01/11 09:00 [medline]
PHST- 2007/07/28 09:00 [entrez]
AID - S0716-97602007000100003 [pii]
AID - 10.4067/s0716-97602007000100003 [doi]
PST - ppublish
SO  - Biol Res. 2007;40(1):23-8. doi: 10.4067/s0716-97602007000100003. Epub 2007 Jul 
      19.