PMID- 17657777
OWN - NLM
STAT- MEDLINE
DCOM- 20071019
LR  - 20071203
IS  - 0006-3592 (Print)
IS  - 0006-3592 (Linking)
VI  - 98
IP  - 1
DP  - 2007 Sep 1
TI  - Design and application of an oscillatory compression device for cell constructs.
PG  - 211-20
AB  - Mechanical compression has been shown to impact cell activity; however a need for 
      a single device to perform a broader range of parametric studies exists. We have 
      developed an oscillatory displacement controlled device to uniaxially strain cell 
      constructs under both static and dynamic compression and used this device to 
      investigate gene expression in cell constructs. The device has a wide stroke 
      (0.25-4 mm) and frequency range (0.1-3 Hz) and several loading waveforms are 
      possible. Alginate cellular constructs with embedded equine chondrocytes were 
      tested and viability was maintained for the 24 h test period. Off-line mechanical 
      testing is described and a modulus value of 18.2 +/- 1.3 kPa found for alginate 
      disks which indicates the level of stress achieved with this deformation profile. 
      Static (15% strain) and dynamic (15% strain, 1 Hz, triangle waveform) testing of 
      chondrocyte constructs was performed and static compression showed significantly 
      higher collagen II expression than dynamic using quantitative RT-PCR. In 
      contrast, differences in matrix metalloproteinase-3 (MMP-3) expression were 
      statistically insignificant. These studies indicate the utility of our device for 
      studying cell activity in response to compression and suggest further studies 
      regarding how the load and strain spectrum impact chondrocyte activity.
FAU - Cassino, Theresa R
AU  - Cassino TR
AD  - Department of Chemical Engineering, Virginia Polytechnic Institute and State 
      University, Virginia, USA.
FAU - Anderson, Roger
AU  - Anderson R
FAU - Love, Brian J
AU  - Love BJ
FAU - Huckle, William R
AU  - Huckle WR
FAU - Seamans, Diane K
AU  - Seamans DK
FAU - Forsten-Williams, Kimberly
AU  - Forsten-Williams K
LA  - eng
GR  - AR46414/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Biotechnol Bioeng
JT  - Biotechnology and bioengineering
JID - 7502021
SB  - IM
MH  - Animals
MH  - Cell Culture Techniques/*instrumentation/methods
MH  - Cell Proliferation
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Chondrocytes/*cytology/*physiology
MH  - Compressive Strength
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Horses
MH  - Mechanotransduction, Cellular/*physiology
MH  - Oscillometry/*instrumentation/methods
MH  - Physical Stimulation/*instrumentation/methods
MH  - Pressure
EDAT- 2007/07/28 09:00
MHDA- 2007/10/20 09:00
CRDT- 2007/07/28 09:00
PHST- 2007/07/28 09:00 [pubmed]
PHST- 2007/10/20 09:00 [medline]
PHST- 2007/07/28 09:00 [entrez]
AID - 10.1002/bit.21422 [doi]
PST - ppublish
SO  - Biotechnol Bioeng. 2007 Sep 1;98(1):211-20. doi: 10.1002/bit.21422.