PMID- 17658411
OWN - NLM
STAT- MEDLINE
DCOM- 20071002
LR  - 20181113
IS  - 0272-2712 (Print)
IS  - 0272-2712 (Linking)
VI  - 27
IP  - 3
DP  - 2007 Sep
TI  - Cellular image analysis and imaging by flow cytometry.
PG  - 653-70, viii
AB  - Imaging flow cytometry combines the statistical power and fluorescence 
      sensitivity of standard flow cytometry with the spatial resolution and 
      quantitative morphology of digital microscopy. The technique is a good fit for 
      clinical applications by providing a convenient means for imaging and analyzing 
      cells directly in bodily fluids. Examples are provided of the discrimination of 
      cancerous from normal mammary epithelial cells and the high-throughput 
      quantitation of fluorescence in situ hybridization (FISH) probes in human 
      peripheral blood mononuclear cells. The FISH application will be enhanced further 
      by the integration of extended depth-of-field imaging technology with the current 
      optical system.
FAU - Basiji, David A
AU  - Basiji DA
AD  - Amnis Corporation, Seattle, 2505 Third Avenue, Suite 210, WA 98121, USA. 
      dbasiji@amnis.com
FAU - Ortyn, William E
AU  - Ortyn WE
FAU - Liang, Luchuan
AU  - Liang L
FAU - Venkatachalam, Vidya
AU  - Venkatachalam V
FAU - Morrissey, Philip
AU  - Morrissey P
LA  - eng
GR  - R43 GM058956-01/GM/NIGMS NIH HHS/United States
GR  - R44 CA001798-02/CA/NCI NIH HHS/United States
GR  - 1 R43 GM58956-01/GM/NIGMS NIH HHS/United States
GR  - 9 R44 CA01798-02/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Clin Lab Med
JT  - Clinics in laboratory medicine
JID - 8100174
SB  - IM
MH  - Breast Neoplasms/immunology/*pathology
MH  - Female
MH  - Flow Cytometry/*methods
MH  - Humans
MH  - Image Cytometry/*methods
MH  - In Situ Hybridization, Fluorescence
MH  - Jurkat Cells/immunology/*pathology
PMC - PMC2034394
MID - NIHMS29623
EDAT- 2007/07/31 09:00
MHDA- 2007/10/03 09:00
PMCR- 2008/09/01
CRDT- 2007/07/31 09:00
PHST- 2007/07/31 09:00 [pubmed]
PHST- 2007/10/03 09:00 [medline]
PHST- 2007/07/31 09:00 [entrez]
PHST- 2008/09/01 00:00 [pmc-release]
AID - S0272-2712(07)00053-4 [pii]
AID - 10.1016/j.cll.2007.05.008 [doi]
PST - ppublish
SO  - Clin Lab Med. 2007 Sep;27(3):653-70, viii. doi: 10.1016/j.cll.2007.05.008.