PMID- 17658593
OWN - NLM
STAT- MEDLINE
DCOM- 20071107
LR  - 20191210
IS  - 0093-691X (Print)
IS  - 0093-691X (Linking)
VI  - 68
IP  - 5
DP  - 2007 Sep 15
TI  - Intrafollicular amino acid concentration and the effect of amino acids in a 
      defined maturation medium on porcine oocyte maturation, fertilization, and 
      preimplantation development.
PG  - 728-35
AB  - The objective of this study was to determine the intrafollicular concentrations 
      of free amino acids in pigs and to examine the effect of amino acids in a 
      chemically defined maturation medium on oocyte maturation, in vitro fertilization 
      (IVF), and embryo development in vitro. Pooled follicular fluid aspirated 
      separately from small (<3mm in diameter), medium (3-8mm), and large follicles 
      (>8mm) in three pairs of ovaries was analyzed for amino acid concentration. In 
      addition, oocyte maturation, fertilization, and embryo development were examined 
      after in vitro maturation (IVM) of oocytes in a defined maturation medium 
      supplemented individually with glutamate (GLU), glutamine (GLN), glycine (GLY), 
      aspartate (ASP), asparagine (ASN), arginine (ARG), alanine (ALA), leucine (LEU), 
      lysine (LYS), proline (PRO), and valine (VAL). Irrespective of follicle size, 
      GLY, GLU, ALA, GLN, and PRO were the most abundant amino acids in pig follicular 
      fluid (pFF). Sperm penetration was not altered by amino acid treatment during 
      IVM, but monospermic fertilization was increased (P<0.05) by GLN, ASP, and VAL. 
      All amino acids except ASP and ASN stimulated (P<0.05) male pronuclear formation 
      after IVF. ARG and ALA treatment during IVM improved (P<0.05) blastocyst 
      formation. In conclusion, GLY, GLU, ALA, GLN, and PRO were the most abundant 
      amino acids in pFF and amino acids in a defined medium improved porcine 
      monospermic fertilization, male pronuclear formation, and preimplantation 
      development.
FAU - Hong, J
AU  - Hong J
AD  - School of Veterinary Medicine and Institute of Veterinary Science, Kangwon 
      National University, Chunchon, Republic of Korea.
FAU - Lee, E
AU  - Lee E
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070720
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 0 (Amino Acids)
RN  - 0 (Culture Media)
SB  - IM
MH  - Amino Acids/*analysis/*pharmacology
MH  - Animals
MH  - Cell Size
MH  - Culture Media/*pharmacology
MH  - Embryo Culture Techniques
MH  - Embryonic Development/*drug effects
MH  - Female
MH  - Fertilization/*drug effects
MH  - Follicular Fluid/*chemistry
MH  - Male
MH  - Oocytes/cytology/*drug effects/metabolism
MH  - Oogenesis/drug effects
MH  - Ovarian Follicle/drug effects
MH  - Swine/*embryology
EDAT- 2007/07/31 09:00
MHDA- 2007/11/08 09:00
CRDT- 2007/07/31 09:00
PHST- 2007/04/02 00:00 [received]
PHST- 2007/06/08 00:00 [accepted]
PHST- 2007/07/31 09:00 [pubmed]
PHST- 2007/11/08 09:00 [medline]
PHST- 2007/07/31 09:00 [entrez]
AID - S0093-691X(07)00328-7 [pii]
AID - 10.1016/j.theriogenology.2007.06.002 [doi]
PST - ppublish
SO  - Theriogenology. 2007 Sep 15;68(5):728-35. doi: 
      10.1016/j.theriogenology.2007.06.002. Epub 2007 Jul 20.