PMID- 17659810
OWN - NLM
STAT- MEDLINE
DCOM- 20080111
LR  - 20220408
IS  - 0169-5002 (Print)
IS  - 1872-8332 (Electronic)
IS  - 0169-5002 (Linking)
VI  - 58
IP  - 2
DP  - 2007 Nov
TI  - DNA methylation profile of 28 potential marker loci in malignant mesothelioma.
PG  - 220-30
AB  - Patients with malignant mesothelioma (MM), an aggressive cancer associated with 
      asbestos exposure, usually present clinically with advanced disease and this 
      greatly reduces the likelihood of curative treatment. MM is difficult to diagnose 
      without invasive techniques; the development of non-invasively detectable 
      molecular markers would therefore be highly beneficial. DNA methylation changes 
      in cancer cells provide powerful markers that are potentially detectable 
      non-invasively in DNA shed into bodily fluids. Here we examined the methylation 
      status of 28 loci in 52 MM tumors to investigate their potential as molecular 
      markers for MM. To exclude candidate MM markers that might be positive in 
      biopsies/pleural fluid due to contaminating surrounding non-tumor lung 
      tissue/DNA, we also examined the methylation of these markers in lung samples 
      (age- or environmentally induced hypermethylation is frequently observed in 
      non-cancerous lung). Statistically significantly increased methylation in MM 
      versus non-tumor lung samples was found for estrogen receptor 1 (ESR1; p = 
      0.0002), solute carrier family 6 member 20 (SLC6A20; p = 0.0022) and spleen 
      tyrosine kinase (SYK; p=0.0003). Examination of associations between methylation 
      levels of the 28 loci and clinical parameters suggest associations of the 
      methylation status of metallothionein genes with gender, histology, asbestos 
      exposure, and lymph node involvement, and the methylation status of leucine 
      zipper tumor suppressor 1 (LZTS1) and SLC6A20 with survival.
FAU - Tsou, Jeffrey A
AU  - Tsou JA
AD  - Norris Cancer Center and Department of Surgery, Keck School of Medicine, 
      University of Southern California, Room NOR6420, 1441 Eastlake Avenue, Los 
      Angeles, CA 90089-9176, USA.
FAU - Galler, Janice S
AU  - Galler JS
FAU - Wali, Anil
AU  - Wali A
FAU - Ye, Wei
AU  - Ye W
FAU - Siegmund, Kimberly D
AU  - Siegmund KD
FAU - Groshen, Susan
AU  - Groshen S
FAU - Laird, Peter W
AU  - Laird PW
FAU - Turla, Sally
AU  - Turla S
FAU - Koss, Michael N
AU  - Koss MN
FAU - Pass, Harvey I
AU  - Pass HI
FAU - Laird-Offringa, Ite A
AU  - Laird-Offringa IA
LA  - eng
GR  - P30 CA014089/CA/NCI NIH HHS/United States
GR  - P30 CA014089-349012/CA/NCI NIH HHS/United States
GR  - P30 CA 14089/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20070730
PL  - Ireland
TA  - Lung Cancer
JT  - Lung cancer (Amsterdam, Netherlands)
JID - 8800805
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Neoplasm Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/*genetics
MH  - Cluster Analysis
MH  - *DNA Methylation
MH  - Female
MH  - Humans
MH  - Kaplan-Meier Estimate
MH  - Male
MH  - Mesothelioma/*genetics
MH  - Middle Aged
MH  - Neoplasm Proteins/genetics
PMC - PMC2752414
MID - NIHMS33001
COIS- Conflict of interest statement I.A.L.-O. and P.W.L. are shareholders of 
      Epigenomics AG, which has a commercial interest in the development of DNA markers 
      for disease detection and diagnosis. None of the work performed in the 
      laboratories of any of the authors is or has been supported by Epigenomics.
EDAT- 2007/07/31 09:00
MHDA- 2008/01/12 09:00
PMCR- 2009/09/25
CRDT- 2007/07/31 09:00
PHST- 2007/02/07 00:00 [received]
PHST- 2007/05/28 00:00 [revised]
PHST- 2007/06/19 00:00 [accepted]
PHST- 2007/07/31 09:00 [pubmed]
PHST- 2008/01/12 09:00 [medline]
PHST- 2007/07/31 09:00 [entrez]
PHST- 2009/09/25 00:00 [pmc-release]
AID - S0169-5002(07)00340-6 [pii]
AID - 10.1016/j.lungcan.2007.06.015 [doi]
PST - ppublish
SO  - Lung Cancer. 2007 Nov;58(2):220-30. doi: 10.1016/j.lungcan.2007.06.015. Epub 2007 
      Jul 30.