PMID- 17660499
OWN - NLM
STAT- MEDLINE
DCOM- 20071214
LR  - 20220129
IS  - 0953-8178 (Print)
IS  - 0953-8178 (Linking)
VI  - 19
IP  - 9
DP  - 2007 Sep
TI  - IL-10 permits transient activation of dendritic cells to tolerize T cells and 
      protect from central nervous system autoimmune disease.
PG  - 1123-34
AB  - Dendritic cells (DCs) are key players in the development of immunity. They can 
      direct both the size and the quality of an immune response and thus are 
      attractive tools to mediate immunotherapy. DC function has been thought to 
      reflect the cells' maturation, with immunosuppressive agents such as IL-10 
      understood to retain DCs in an immature and tolerogenic state. Here we report 
      that DC activated in the presence of IL-10 do show functional and phenotypic 
      maturation. Their activation is transient and occurs earlier and more briefly 
      than in cells matured with LPS alone. Despite initially equivalent up-regulation 
      of surface MHC and co-stimulation, the IL-10-treated DCs expressed little IL-12 
      and failed to stimulate T cell proliferation both in vitro and in vivo. 
      Interaction with IL-10-treated DCs rendered antigen-specific T cells unresponsive 
      to subsequent challenge and their injection reduced the severity of experimental 
      autoimmune disease. Our data suggest that IL-10 acts not by inhibiting maturation 
      but instead by controlling the kinetics and the quality of DC activation. This 
      alternative pathway of DC differentiation offers significant therapeutic promise.
FAU - Perona-Wright, Georgia
AU  - Perona-Wright G
AD  - Institute of Immunology and Infection Research, School of Biological Sciences, 
      University of Edinburgh, Edinburgh, Scotland, UK.
FAU - Anderton, Stephen M
AU  - Anderton SM
FAU - Howie, Sarah E M
AU  - Howie SE
FAU - Gray, David
AU  - Gray D
LA  - eng
GR  - G117/515/MRC_/Medical Research Council/United Kingdom
GR  - 068937/WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070728
PL  - England
TA  - Int Immunol
JT  - International immunology
JID - 8916182
RN  - 0 (Lipopolysaccharides)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/drug effects/immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/*drug effects/immunology
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Immune Tolerance/*drug effects
MH  - Immunophenotyping
MH  - Interleukin-10/physiology/*therapeutic use
MH  - Interleukin-12/biosynthesis
MH  - Lipopolysaccharides/pharmacology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Multiple Sclerosis/*drug therapy/immunology
MH  - T-Lymphocytes/*drug effects/immunology
MH  - Time Factors
EDAT- 2007/07/31 09:00
MHDA- 2007/12/15 09:00
CRDT- 2007/07/31 09:00
PHST- 2007/07/31 09:00 [pubmed]
PHST- 2007/12/15 09:00 [medline]
PHST- 2007/07/31 09:00 [entrez]
AID - dxm084 [pii]
AID - 10.1093/intimm/dxm084 [doi]
PST - ppublish
SO  - Int Immunol. 2007 Sep;19(9):1123-34. doi: 10.1093/intimm/dxm084. Epub 2007 Jul 
      28.