PMID- 17660838
OWN - NLM
STAT- MEDLINE
DCOM- 20071024
LR  - 20070919
IS  - 0268-3369 (Print)
IS  - 0268-3369 (Linking)
VI  - 40
IP  - 7
DP  - 2007 Oct
TI  - Marrow graft rejection by repeated transfusions of allogeneic donor spleen cells.
PG  - 691-8
AB  - Many hematological diseases require long-term transfusion support, which causes 
      production of donor-reactive antibodies in sensitized recipients. Sensitized 
      patients are at an increased risk for graft rejection when they undergo 
      allogeneic hematopoietic stem cell transplantation (allo-HSCT). Here, we 
      established a highly sensitized murine model to investigate the mechanism of 
      donor graft rejection. After BALB/c mice were repeatedly transfused with 
      allogeneic spleen cells from C57BL/6 mice, there was a significant increase in 
      complement-dependent cytotoxicity in the serum of sensitized mice. For 
      transplantation, 1 x 10(7) bone marrow cells (BMCs) from C57BL/6 mice were 
      injected into lethally irradiated recipient BALB/c mice. Sensitized mice died 
      between 12 and 15 days post-transplantation, while non-sensitized mice remained 
      alive after 28 days. The hematopoietic recovery rate declined over time in 
      sensitized recipients. The homing trace assay showed a rapid disappearance of 
      donor BMCs in the spleen and bone marrow of sensitized recipients. In addition, 
      the recipient cells and antibodies in the sensitized serum were capable of 
      inducing high level of cell- and complement-mediated cytotoxicity to the donor 
      graft. Our finding may explain the impaired hematopoietic stem cell homing and 
      poor hematopoietic engraftment observed in highly sensitized allo-HSCT patients.
FAU - Xu, L H
AU  - Xu LH
AD  - Department of Pediatrics, Second Affiliated Hospital of Zhongshan University, 
      Guangzhou, PR China.
FAU - Fang, J P
AU  - Fang JP
FAU - Huang, W G
AU  - Huang WG
FAU - Xu, H G
AU  - Xu HG
FAU - Weng, W J
AU  - Weng WJ
FAU - Kao, G S
AU  - Kao GS
FAU - Le, Y
AU  - Le Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070730
PL  - England
TA  - Bone Marrow Transplant
JT  - Bone marrow transplantation
JID - 8702459
RN  - 0 (Isoantibodies)
SB  - IM
MH  - Animals
MH  - Bone Marrow Transplantation/*immunology/mortality
MH  - Cell Transplantation/*adverse effects
MH  - Graft Rejection/*etiology
MH  - Humans
MH  - Isoantibodies/blood
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Models, Animal
MH  - Spleen/*cytology
MH  - Tissue Donors
MH  - Transplantation, Homologous
EDAT- 2007/07/31 09:00
MHDA- 2007/10/25 09:00
CRDT- 2007/07/31 09:00
PHST- 2007/07/31 09:00 [pubmed]
PHST- 2007/10/25 09:00 [medline]
PHST- 2007/07/31 09:00 [entrez]
AID - 1705791 [pii]
AID - 10.1038/sj.bmt.1705791 [doi]
PST - ppublish
SO  - Bone Marrow Transplant. 2007 Oct;40(7):691-8. doi: 10.1038/sj.bmt.1705791. Epub 
      2007 Jul 30.