PMID- 17660953
OWN - NLM
STAT- MEDLINE
DCOM- 20080129
LR  - 20231120
IS  - 0300-8177 (Print)
IS  - 1573-4919 (Electronic)
IS  - 0300-8177 (Linking)
VI  - 306
IP  - 1-2
DP  - 2007 Dec
TI  - Selection of DNA aptamers against DC-SIGN protein.
PG  - 71-7
AB  - Dendritic cells (DCs) are professional antigen-presenting cells and have come to 
      be appreciated as critical controllers of the immune response, especially T cell 
      responses. Apart from presenting antigens to T cells, DCs carry out many other 
      functions in regulating immunity. DC-specific intercellular adhesion molecule 
      (ICAM)-3 grabbing non-integrin (DC-SIGN) is a novel receptor that plays an 
      important role in DC migration and adhesion, the inflammatory response, T cell 
      activation, initiating the immune response, and immune escape of pathogens and 
      tumors. DC-SIGN mediates DC binding to ICAM-3 on the T cell surface and ICAM-2 on 
      the endothelial cell (EC) surface, and takes part in the initial interaction 
      between DC and T cells or vascular ECs. The procedure of systematic evolution of 
      ligands by exponential enrichment (SELEX) is a method in which single-stranded 
      oligonucleotides are selected from a wide variety of sequences, based on their 
      interaction with a target molecule. In this study, we selected DNA aptamers 
      against DC-SIGN protein by SELEX, and measured their binding affinity for 
      DC-SIGN. Finally, an appropriate aptamer with high affinity for DC-SIGN was 
      obtained, and it blocked DC adhesion to ECs as effectively as anti-DC-SIGN 
      monoclonal antibody.
FAU - Hui, Yan
AU  - Hui Y
AD  - Department of Cardiology, First Affiliated Hospital, Zhejiang University School 
      of Medicine, No. 79, Qingchun Road, Hangzhou 310003, China.
FAU - Shan, Li
AU  - Shan L
FAU - Lin-Fu, Zhou
AU  - Lin-Fu Z
FAU - Jian-Hua, Zhu
AU  - Jian-Hua Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070728
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (DNA, Single-Stranded)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Amino Acid Sequence
MH  - Aptamers, Nucleotide/*genetics/metabolism
MH  - Cell Adhesion/physiology
MH  - Cell Adhesion Molecules/*genetics
MH  - DNA, Single-Stranded/*genetics/metabolism
MH  - Dendritic Cells/immunology/metabolism
MH  - Endothelium, Vascular/metabolism
MH  - Humans
MH  - Lectins, C-Type/*genetics
MH  - Molecular Sequence Data
MH  - Receptors, Cell Surface/*genetics
MH  - Sequence Homology, Amino Acid
PMC - PMC7089390
EDAT- 2007/07/31 09:00
MHDA- 2008/01/30 09:00
PMCR- 2020/03/23
CRDT- 2007/07/31 09:00
PHST- 2007/04/16 00:00 [received]
PHST- 2007/07/12 00:00 [accepted]
PHST- 2007/07/31 09:00 [pubmed]
PHST- 2008/01/30 09:00 [medline]
PHST- 2007/07/31 09:00 [entrez]
PHST- 2020/03/23 00:00 [pmc-release]
AID - 9555 [pii]
AID - 10.1007/s11010-007-9555-x [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2007 Dec;306(1-2):71-7. doi: 10.1007/s11010-007-9555-x. Epub 
      2007 Jul 28.