PMID- 17661338
OWN - NLM
STAT- MEDLINE
DCOM- 20120306
LR  - 20191210
IS  - 0951-4198 (Print)
IS  - 0951-4198 (Linking)
VI  - 21
IP  - 17
DP  - 2007
TI  - Monitoring quinolone antibacterial residues in bovine tissues: extraction with 
      hot water and liquid chromatography coupled to a single- or triple-quadrupole 
      mass spectrometer.
PG  - 2833-42
AB  - A rapid and sensitive procedure for determining residues of seven quinolone 
      antibacterials in bovine muscle, kidney and liver is presented. The method is 
      based on the matrix solid-phase dispersion (MSPD) technique with hot water as 
      extractant followed by liquid chromatography/single quadrupole mass spectrometry 
      (LC/MS) or triple-quadrupole mass spectrometry (LC/MS/MS). After dispersing 
      tissue samples on hydrazine sulfate treated sand, target compounds were eluted 
      from the MSPD column by passing through it 4 mL of water heated at 100 degrees C. 
      After pH adjustment and filtration, 200 and 5 microL of the aqueous extracts were 
      respectively injected into the LC/MS and LC/MS/MS instruments. With the former 
      instrument, MS data were acquired in the three-ion selected ion monitoring mode, 
      while MS/MS data acquisition was performed in the multi-reaction monitoring mode 
      by selecting two precursor ion to product ion transitions for each target 
      compound. Hot water appeared to be an efficient extracting medium, since absolute 
      recoveries of the analytes were 84-102%. Using norfloxacin (a quinolone not used 
      in veterinary medicine) as surrogate internal standard, the accuracy of the 
      method at three concentration levels equal to 0.5, 1 and 1.5 times the maximum 
      residue limits (MRLs) set by the european union was 88-109% with relative 
      standard deviations (RSDs) not higher than 7%. The use of LC/MS/MS allowed 
      detection and quantification of the analytes in any tissue considered to be 
      performed at concentrations by far lower than half of their MRLs. Vice versa, the 
      single-quadrupole MS arrangement, while succeeding in monitoring quinolones in 
      muscle tissue at the 0.5 MRL level, showed to be not sufficiently selective for 
      unambiguous identification of some quinolones in kidney and liver.
FAU - Bogialli, Sara
AU  - Bogialli S
AD  - Dipartimento di Chimica, Universita La Sapienza, Piazza Aldo Moro 5, 00185 Roma, 
      Italy. sara.bogialli@uniromal.it
FAU - D'Ascenzo, Giuseppe
AU  - D'Ascenzo G
FAU - Di Corcia, Antonio
AU  - Di Corcia A
FAU - Innocenti, Giorgiana
AU  - Innocenti G
FAU - Lagana, Aldo
AU  - Lagana A
FAU - Pacchiarotta, Tiziana
AU  - Pacchiarotta T
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - England
TA  - Rapid Commun Mass Spectrom
JT  - Rapid communications in mass spectrometry : RCM
JID - 8802365
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Quinolones)
RN  - 059QF0KO0R (Water)
SB  - IM
MH  - Anti-Bacterial Agents/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Food Analysis/*methods
MH  - Food Contamination/*analysis
MH  - Hot Temperature
MH  - Meat/*analysis
MH  - Quinolones/*analysis
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Solid Phase Microextraction/methods
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Water/chemistry
EDAT- 2007/07/31 09:00
MHDA- 2012/03/07 06:00
CRDT- 2007/07/31 09:00
PHST- 2007/07/31 09:00 [pubmed]
PHST- 2012/03/07 06:00 [medline]
PHST- 2007/07/31 09:00 [entrez]
AID - 10.1002/rcm.3155 [doi]
PST - ppublish
SO  - Rapid Commun Mass Spectrom. 2007;21(17):2833-42. doi: 10.1002/rcm.3155.