PMID- 17662155
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20070905
LR  - 20200928
IS  - 1476-8518 (Electronic)
IS  - 1476-8518 (Linking)
VI  - 5
DP  - 2007 Jul 29
TI  - CTLA-4 blockade during dendritic cell based booster vaccination influences 
      dendritic cell survival and CTL expansion.
PG  - 9
AB  - Dendritic cells (DCs) are potent antigen-presenting cells and critical for the 
      priming of CD8+ T cells. Therefore the use of these cells as adjuvant cells has 
      been tested in a large number of experimental and clinical vaccination studies, 
      in particular cancer vaccine studies. A number of protocols are emerging that 
      combine vaccination with CTL expanding strategies, such as e.g. blockade of 
      CTLA-4 signalling. On the other hand, the lifespan and in vivo survival of 
      therapeutic DCs have only been addressed in a few studies, although this is of 
      importance for the kinetics of CTL induction during vaccination. We have 
      previously reported that DCs loaded with specific antigens are eliminated by 
      antigen specific CTLs in vivo and that this elimination affects the potential for 
      in vivo CTL generation. We now show that CTLA-4 blockade increases the number of 
      DC vaccine induced LCMV gp33 specific CTLs and the lysis of relevant in vivo 
      targets. However, the CTLA-4 blockage dependent expansion of CTLs also affect DC 
      survival during booster DC injections and our data suggest that during a booster 
      DC vaccine, the largest increase in CTL levels is already obtained during the 
      first vaccination.
FAU - Pedersen, Anders E
AU  - Pedersen AE
AD  - Department of International Health, Immunology and Microbiology, The Panum 
      Institute, University of Copenhagen, Denmark. elmpedersen@hotmail.com
FAU - Ronchese, Franca
AU  - Ronchese F
LA  - eng
PT  - Journal Article
DEP - 20070729
PL  - England
TA  - J Immune Based Ther Vaccines
JT  - Journal of immune based therapies and vaccines
JID - 101152206
PMC - PMC1950502
EDAT- 2007/07/31 09:00
MHDA- 2007/07/31 09:01
PMCR- 2007/07/29
CRDT- 2007/07/31 09:00
PHST- 2007/05/10 00:00 [received]
PHST- 2007/07/29 00:00 [accepted]
PHST- 2007/07/31 09:00 [pubmed]
PHST- 2007/07/31 09:01 [medline]
PHST- 2007/07/31 09:00 [entrez]
PHST- 2007/07/29 00:00 [pmc-release]
AID - 1476-8518-5-9 [pii]
AID - 10.1186/1476-8518-5-9 [doi]
PST - epublish
SO  - J Immune Based Ther Vaccines. 2007 Jul 29;5:9. doi: 10.1186/1476-8518-5-9.