PMID- 17662836
OWN - NLM
STAT- MEDLINE
DCOM- 20070914
LR  - 20231127
IS  - 0076-6879 (Print)
IS  - 0076-6879 (Linking)
VI  - 424
DP  - 2007
TI  - RNA editing uridylyltransferases of trypanosomatids.
PG  - 55-73
AB  - Terminal RNA uridylyltransferases (TUTases) catalyze the transfer of UMP residues 
      to the 3' hydroxyl group of RNA. These enzymes belong to the DNA polymerase beta 
      superfamily, which also includes poly(A) polymerases, CCA-adding enzymes, and 
      other nucleotidyltransferases. Studies of uridylyl insertion/deletion RNA editing 
      in mitochondria of trypanosomatids provided the first examples of biological 
      functions for TUTases: posttranscriptional uridylylation of guide RNAs by RNA 
      editing TUTase 1 (RET1) and U-insertion mRNA editing by RNA editing TUTase 2 
      (RET2). The editing TUTases are unified by the presence of conserved catalytic 
      and nucleotide base recognition domains, yet differ substantially in auxiliary 
      function-specific domains, quaternary structure, RNA substrate specificity, and 
      processivity. This chapter describes isolation of TUTases and their complexes 
      from trypanosomatids, methods used for analysis of interactions involving RET1 
      and RET2, purification of recombinant proteins, and enzyme kinetic assays.
FAU - Aphasizhev, Ruslan
AU  - Aphasizhev R
AD  - Department of Microbiology and Molecular Genetics, University of California, 
      Irvine, California, USA.
FAU - Aphasizheva, Inna
AU  - Aphasizheva I
LA  - eng
GR  - AI064653/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Methods Enzymol
JT  - Methods in enzymology
JID - 0212271
RN  - 0 (Protozoan Proteins)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.7.- (RNA Nucleotidyltransferases)
RN  - EC 2.7.7.- (UTP-RNA uridylyltransferase)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
SB  - IM
MH  - Animals
MH  - Biochemistry/methods
MH  - Chromatography
MH  - Leishmania/*metabolism
MH  - Proteasome Endopeptidase Complex/metabolism
MH  - Protein Interaction Mapping
MH  - Protozoan Proteins/metabolism
MH  - *RNA Editing
MH  - RNA Nucleotidyltransferases/chemistry/genetics/*metabolism
MH  - RNA Processing, Post-Transcriptional
MH  - Recombinant Proteins/metabolism
MH  - Trypanosoma brucei brucei/*metabolism
EDAT- 2007/07/31 09:00
MHDA- 2007/09/15 09:00
CRDT- 2007/07/31 09:00
PHST- 2007/07/31 09:00 [pubmed]
PHST- 2007/09/15 09:00 [medline]
PHST- 2007/07/31 09:00 [entrez]
AID - S0076-6879(07)24003-0 [pii]
AID - 10.1016/S0076-6879(07)24003-0 [doi]
PST - ppublish
SO  - Methods Enzymol. 2007;424:55-73. doi: 10.1016/S0076-6879(07)24003-0.