PMID- 17662941
OWN - NLM
STAT- MEDLINE
DCOM- 20070913
LR  - 20181113
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 130
IP  - 2
DP  - 2007 Jul 27
TI  - BLM ortholog, Sgs1, prevents aberrant crossing-over by suppressing formation of 
      multichromatid joint molecules.
PG  - 259-72
AB  - Bloom's helicase (BLM) is thought to prevent crossing-over during DNA 
      double-strand-break repair (DSBR) by disassembling double-Holliday junctions 
      (dHJs) or by preventing their formation. We show that the Saccharomyces 
      cerevisiae BLM ortholog, Sgs1, prevents aberrant crossing-over during meiosis by 
      suppressing formation of joint molecules (JMs) comprising three and four 
      interconnected duplexes. Sgs1 and procrossover factors, Msh5 and Mlh3, are 
      antagonistic since Sgs1 prevents dHJ formation in msh5 cells and sgs1 mutation 
      alleviates crossover defects of both msh5 and mlh3 mutants. We propose that 
      differential activity of Sgs1 and procrossover factors at the two DSB ends 
      effects productive formation of dHJs and crossovers and prevents multichromatid 
      JMs and counterproductive crossing-over. Strand invasion of different templates 
      by both DSB ends may be a common feature of DSBR that increases repair efficiency 
      but also the likelihood of associated crossing-over. Thus, by disrupting aberrant 
      JMs, BLM-related helicases maximize repair efficiency while minimizing the risk 
      of deleterious crossing-over.
FAU - Oh, Steve D
AU  - Oh SD
AD  - Section of Microbiology, University of California, Davis, One Shields Avenue, 
      Davis, CA 95616, USA.
FAU - Lao, Jessica P
AU  - Lao JP
FAU - Hwang, Patty Yi-Hwa
AU  - Hwang PY
FAU - Taylor, Andrew F
AU  - Taylor AF
FAU - Smith, Gerald R
AU  - Smith GR
FAU - Hunter, Neil
AU  - Hunter N
LA  - eng
GR  - R01 GM031693-25/GM/NIGMS NIH HHS/United States
GR  - GM031693/GM/NIGMS NIH HHS/United States
GR  - GM074223/GM/NIGMS NIH HHS/United States
GR  - R01 GM031693/GM/NIGMS NIH HHS/United States
GR  - R56 GM031693/GM/NIGMS NIH HHS/United States
GR  - R01 GM074223/GM/NIGMS NIH HHS/United States
GR  - R01 GM074223-03/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (DNA, Cruciform)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (NDT80 protein, S cerevisiae)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
RN  - EC 3.6.1.- (Bloom syndrome protein)
RN  - EC 3.6.1.- (SGS1 protein, S cerevisiae)
RN  - EC 3.6.4.- (DNA Helicases)
RN  - EC 3.6.4.12 (RecQ Helicases)
SB  - IM
MH  - Adenosine Triphosphatases/*chemistry
MH  - Chromatids/*metabolism/ultrastructure
MH  - Crossing Over, Genetic/*genetics
MH  - DNA Breaks, Double-Stranded
MH  - DNA Helicases/*chemistry
MH  - DNA, Cruciform/metabolism
MH  - DNA-Binding Proteins/metabolism
MH  - Meiosis
MH  - Models, Genetic
MH  - Molecular Weight
MH  - Mutation/genetics
MH  - RecQ Helicases/*metabolism
MH  - Saccharomyces cerevisiae/cytology/*genetics
MH  - Saccharomyces cerevisiae Proteins/*metabolism
MH  - *Sequence Homology, Amino Acid
MH  - Sister Chromatid Exchange
MH  - Transcription Factors/metabolism
PMC - PMC2034285
MID - NIHMS28046
EDAT- 2007/07/31 09:00
MHDA- 2007/09/14 09:00
PMCR- 2008/07/27
CRDT- 2007/07/31 09:00
PHST- 2007/01/10 00:00 [received]
PHST- 2007/04/18 00:00 [revised]
PHST- 2007/05/15 00:00 [accepted]
PHST- 2007/07/31 09:00 [pubmed]
PHST- 2007/09/14 09:00 [medline]
PHST- 2007/07/31 09:00 [entrez]
PHST- 2008/07/27 00:00 [pmc-release]
AID - S0092-8674(07)00674-5 [pii]
AID - 10.1016/j.cell.2007.05.035 [doi]
PST - ppublish
SO  - Cell. 2007 Jul 27;130(2):259-72. doi: 10.1016/j.cell.2007.05.035.