PMID- 17663497
OWN - NLM
STAT- MEDLINE
DCOM- 20120306
LR  - 20070808
IS  - 0951-4198 (Print)
IS  - 0951-4198 (Linking)
VI  - 21
IP  - 17
DP  - 2007
TI  - Matrix-assisted laser desorption/ionization time-of-flight based wheat gliadin 
      protein peaks are useful molecular markers for wheat genetic study.
PG  - 2913-7
AB  - Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) 
      instrumentation has been used to analyze wheat seed gliadins as an alternative to 
      other established methods, including sodium dodecyl sulfate polyacrylamide gel 
      electrophoresis (SDS-PAGE), capillary electrophoresis (CE), high-performance 
      liquid chromatography (HPLC), etc. The MALDI-TOF approach has shown to have many 
      advantages such as high resolution, cost effectiveness and high throughput. 
      MALDI-TOF-based gliadin profiles have been used for fast wheat cultivar 
      identification. However, the genetic information represented by individual 
      gliadin peaks has not been utilized. In this study a wheat doubled haploid 
      population with a genetic linkage map of good coverage was used to assay 
      individual gliadin peaks from MALDI-TOF profiles as molecular markers. Eight 
      segregating peaks in the population were scored as polymorphic across the 
      population. The 1 to 1 segregating ratios validated the scoring of the peaks and 
      all peaks were mapped to the expected chromosomes or linkage groups on the 
      available linkage map: 1 peak on chromosome 1A, 1 on 6A, 4 on 6B and 2 on 6D.
FAU - Chen, J
AU  - Chen J
AD  - Molecular Plant Breeding CRC, Centre for Food and Genomic Medicine, State 
      Agriculture Biotechnology Centre, Murdoch University, Western Australian 
      Department of Agriculture and Food, South Street, Perth, WA 6150 Australia.
FAU - Lan, P
AU  - Lan P
FAU - Tarr, A
AU  - Tarr A
FAU - Yan, Y M
AU  - Yan YM
FAU - Francki, M
AU  - Francki M
FAU - Appels, R
AU  - Appels R
FAU - Ma, W
AU  - Ma W
LA  - eng
PT  - Journal Article
PL  - England
TA  - Rapid Commun Mass Spectrom
JT  - Rapid communications in mass spectrometry : RCM
JID - 8802365
RN  - 0 (Genetic Markers)
RN  - 9007-90-3 (Gliadin)
SB  - IM
MH  - Genetic Markers/*genetics
MH  - Gliadin/*chemistry
MH  - Peptide Mapping/methods
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
MH  - Triticum/*chemistry/*genetics
EDAT- 2007/08/01 09:00
MHDA- 2012/03/07 06:00
CRDT- 2007/08/01 09:00
PHST- 2007/08/01 09:00 [pubmed]
PHST- 2012/03/07 06:00 [medline]
PHST- 2007/08/01 09:00 [entrez]
AID - 10.1002/rcm.3151 [doi]
PST - ppublish
SO  - Rapid Commun Mass Spectrom. 2007;21(17):2913-7. doi: 10.1002/rcm.3151.