PMID- 17663564
OWN - NLM
STAT- MEDLINE
DCOM- 20071120
LR  - 20191210
IS  - 0021-8561 (Print)
IS  - 0021-8561 (Linking)
VI  - 55
IP  - 17
DP  - 2007 Aug 22
TI  - Determination of 2-methylisoborneol and geosmin produced by Streptomyces sp. and 
      Anabaena PCC7120.
PG  - 6823-8
AB  - A new sample preparation and enrichment technique, headspace liquid-phase 
      microextraction (HS-LPME) linked to gas chromatography-mass spectrometry (GC-MS), 
      was developed for the determination of the off-flavor odorants, 
      2-methylisoborneol and geosmin, produced by Streptomyces sp. and Anabaena 
      PCC7120. Some of the factors that influence the extraction efficiency of HS-LPME, 
      such as the type of extraction solvent, ionic strength of sample solution, and 
      sample agitation rate, were studied and optimized by a single factor test. Other 
      factors, including extraction temperature, extraction time, microdrop volume, and 
      headspace volume were optimized by orthogonal array design. Extraction of 
      2-methylisoborneol and geosmin was conducted by exposing 2.5 microL of 1-hexanol 
      for 9 min at 50 degrees C in the headspace of a 20 mL vial with a 10 mL of sample 
      solution saturated by NaCl and stirred at 800 rpm. The developed protocol 
      demonstrated good repeatability (relative standard deviations (RSDs) < 5%), wide 
      linear ranges (10-5000 ng/L, r2 > 0.999), and low limits of detection (LODs) for 
      2-methylisoborneol and geosmin (0.05 ng/L for both analytes). Subsequently, the 
      method was successfully applied to extract the analytes in bacterial cultures 
      with high recoveries (from 94% to 98%). Compared with headspace solid-phase 
      microextraction (HS-SPME), HS-LPME demonstrates better linearity, precision, and 
      recovery. Importantly, the sensitivity is about 1 order of magnitude higher than 
      that of most HS-SPME. The results showed that HS-LPME coupled with GC-MS is a 
      simple, convenient, rapid, sensitive, and effective method for the qualitative 
      and quantitative analysis of 2-methylisoborneol and geosmin.
FAU - Xie, Yuqun
AU  - Xie Y
AD  - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural 
      University, Wuhan, Hubei 430070, People's Republic of China. 
      yuqunxie2007@yahoo.com
FAU - He, Jin
AU  - He J
FAU - Huang, Jun
AU  - Huang J
FAU - Zhang, Jibin
AU  - Zhang J
FAU - Yu, Ziniu
AU  - Yu Z
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070731
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Camphanes)
RN  - 0 (Naphthols)
RN  - 2371-42-8 (2-methylisoborneol)
RN  - MYW912WXJ4 (geosmin)
SB  - IM
MH  - Anabaena/*metabolism
MH  - Camphanes/*analysis/metabolism
MH  - Gas Chromatography-Mass Spectrometry
MH  - Naphthols/*analysis/metabolism
MH  - Sensitivity and Specificity
MH  - Streptomyces/*metabolism
EDAT- 2007/08/01 09:00
MHDA- 2007/12/06 09:00
CRDT- 2007/08/01 09:00
PHST- 2007/08/01 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/08/01 09:00 [entrez]
AID - 10.1021/jf070125o [doi]
PST - ppublish
SO  - J Agric Food Chem. 2007 Aug 22;55(17):6823-8. doi: 10.1021/jf070125o. Epub 2007 
      Jul 31.