PMID- 17664256
OWN - NLM
STAT- MEDLINE
DCOM- 20080414
LR  - 20070830
IS  - 1360-9947 (Print)
IS  - 1360-9947 (Linking)
VI  - 13
IP  - 9
DP  - 2007 Sep
TI  - Differential expression of the metalloproteinase MMP3 and the alpha5 integrin 
      subunit in human myometrium at labour.
PG  - 655-61
AB  - Extensive tissue remodelling occurs in the human myometrium before, during and 
      after parturition. The aim of this study was to investigate the expression of two 
      tissue remodelling molecules, matrix metalloproteinase 3 (MMP3) and alpha5 
      integrin (ITGA5) subunit in human myometrium, during pregnancy and labour. mRNA 
      and protein were isolated from human pregnant labouring and non-labouring 
      myometrial tissue, and also from human primary uterine smooth muscle cells 
      (SMCs). Semi-quantitative RT-PCR, real-time fluorescence RT-PCR and western 
      blotting were subsequently performed to determine the expression levels of MMP3 
      and ITGA5 in the myometrial tissues during pregnancy and labour, and in the 
      primary uterine SMCs. The expression of MMP3 and ITGA5 mRNA and protein are 
      reported for the first time during pregnancy and labour in human myometrium. 
      Furthermore, a significant increase in expression of MMP3 mRNA (41-fold, P = 
      0.001), and a significant decrease in ITGA5 mRNA expression (4-fold, P < 0.001) 
      at labour, were observed. Protein expression of these two molecules was also 
      altered at labour, pro-MMP3 protein expression significantly increased while 
      ITGA5 protein expression decreased, with labour onset. Expression of these 
      molecules was also observed in primary cultured human uterine SMCs. The 
      differential expression of these two tissue remodelling molecules at labour and 
      their detection in uterine SMCs highlights their potential importance in 
      myometrial function during pregnancy, labour and post-partum.
FAU - O'Brien, Margaret
AU  - O'Brien M
AD  - National Centre for Biomedical and Engineering Science, Orbsen Building, National 
      University of Ireland Galway, Galway, Ireland. margaret.obrien@nuigalway.ie
FAU - O'Shaughnessy, Deirdre
AU  - O'Shaughnessy D
FAU - Ahamide, Elodie
AU  - Ahamide E
FAU - Morrison, John J
AU  - Morrison JJ
FAU - Smith, Terry J
AU  - Smith TJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070730
PL  - England
TA  - Mol Hum Reprod
JT  - Molecular human reproduction
JID - 9513710
RN  - 0 (Integrin alpha5)
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Female
MH  - *Gene Expression Profiling
MH  - Humans
MH  - Integrin alpha5/*genetics/metabolism
MH  - Labor, Obstetric
MH  - Matrix Metalloproteinase 3/*genetics/metabolism
MH  - Myometrium/*metabolism
MH  - Pregnancy
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2007/08/01 09:00
MHDA- 2008/04/15 09:00
CRDT- 2007/08/01 09:00
PHST- 2007/08/01 09:00 [pubmed]
PHST- 2008/04/15 09:00 [medline]
PHST- 2007/08/01 09:00 [entrez]
AID - gam047 [pii]
AID - 10.1093/molehr/gam047 [doi]
PST - ppublish
SO  - Mol Hum Reprod. 2007 Sep;13(9):655-61. doi: 10.1093/molehr/gam047. Epub 2007 Jul 
      30.