PMID- 17669165
OWN - NLM
STAT- MEDLINE
DCOM- 20080225
LR  - 20070802
IS  - 1066-5234 (Print)
IS  - 1066-5234 (Linking)
VI  - 54
IP  - 4
DP  - 2007 Jul-Aug
TI  - Differentiation of two very similar glaucomid ciliate morphospecies (Ciliophora, 
      Tetrahymenida) by fluorescence in situ hybridization with 18S rRNA targeted 
      oligonucleotide probes.
PG  - 381-7
AB  - Conventional, morphological identification of ciliates and other protozoa needs 
      considerable experience and often is difficult as various staining methods must 
      be applied. New molecular techniques, such as fluorescence in situ hybridization 
      (FISH) with gene probes, are powerful means to overcome this problem. As a test 
      case, the morphology of two very similar, and thus difficult to differentiate 
      ciliate morphospecies, Glaucoma scintillans and Glaucomides bromelicola, were 
      compared. They were then distinguished by applying the Ciliate-FISH technique 
      with a set of eight 18S rRNA targeted oligonucleotide probes, four of which have 
      been developed for specific detection of G. scintillans. The remaining four 
      probes were designed to detect G. bromelicola in order to prove probe 
      specificities by binding to the homologous target region of the probes mentioned 
      before. The tests resulted in a clear and easy differentiation of the two species 
      by fluorescence signals of three of the four tested probe pairs. Thus, FISH 
      techniques are very useful for the identification and detection of protozoa and 
      might be of great help studying geographical distributions of known taxa.
FAU - Fried, Johannes
AU  - Fried J
AD  - Technische Universitat Munchen, Lehrstuhl fur Mikrobiologie, Am Hochanger 4, 
      85350 Freising, Germany. johannes.fried@uibk.ac.at
FAU - Foissner, Wilhelm
AU  - Foissner W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Eukaryot Microbiol
JT  - The Journal of eukaryotic microbiology
JID - 9306405
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 18S)
SB  - IM
MH  - Animals
MH  - Ciliophora/*classification/genetics/isolation & purification
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Nucleic Acid Hybridization
MH  - Oligonucleotide Probes
MH  - RNA, Ribosomal/genetics
MH  - RNA, Ribosomal, 18S/*analysis/genetics
EDAT- 2007/08/03 09:00
MHDA- 2008/02/26 09:00
CRDT- 2007/08/03 09:00
PHST- 2007/08/03 09:00 [pubmed]
PHST- 2008/02/26 09:00 [medline]
PHST- 2007/08/03 09:00 [entrez]
AID - JEU277 [pii]
AID - 10.1111/j.1550-7408.2007.00277.x [doi]
PST - ppublish
SO  - J Eukaryot Microbiol. 2007 Jul-Aug;54(4):381-7. doi: 
      10.1111/j.1550-7408.2007.00277.x.