PMID- 17669700
OWN - NLM
STAT- MEDLINE
DCOM- 20071101
LR  - 20161126
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1774
IP  - 9
DP  - 2007 Sep
TI  - Purification and antimicrobial activity studies of the N-terminal fragment of 
      ubiquitin from human amniotic fluid.
PG  - 1221-6
AB  - A 4.3-kDa antimicrobial peptide was isolated from human amniotic fluid by 
      dialysis, ultrafiltration, and C18 reversed-phase high performance liquid 
      chromatography. This peptide, which we named Amniotic Fluid Peptide-1 (AFP-1), 
      possessed antimicrobial activity but lacked hemolytic activity. In addition, 
      AFP-1 potently inhibited the growth of a variety of bacteria (Escherichia coli, 
      Salmonella typhimurium, Listeria monocytogenes and Staphylococcus aureus), 
      filamentous fungi (Botrytis cinerea, Aspergillus fumigatus, Neurospora crassa and 
      Fusarium oxysporum) and yeast cells (Candida albicans and Cryptococcus 
      neoformans). Automated Edman degradation showed that the N-terminal sequence of 
      AFP-1 was 
      NH(2)-Met-Gln-Ile-Phe-Val-Lys-Thr-Leu-Thr-Gly-Lys-Thr-Ile-Thr-Leu-Glu-Val-Glu-. 
      The partial sequence had 100% homology to the N-terminal sequence of ubiquitin. 
      Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass 
      spectrometry revealed that the molecular mass of AFP-1 was 4280.2 Da. Our data 
      show an antimicrobial activity of ubiquitin N-terminal derived peptide that makes 
      it suitable for use as an antimicrobial agent.
FAU - Kim, Jin-Young
AU  - Kim JY
AD  - Research Center for Proteineous Materials, Chosun University, Kwangju 501-759, 
      Korea.
FAU - Lee, Sun Young
AU  - Lee SY
FAU - Park, Seong-Cheol
AU  - Park SC
FAU - Shin, Song Yub
AU  - Shin SY
FAU - Choi, Sang Joon
AU  - Choi SJ
FAU - Park, Yoonkyung
AU  - Park Y
FAU - Hahm, Kyung-Soo
AU  - Hahm KS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070710
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Peptide Fragments)
RN  - 0 (Ubiquitin)
RN  - 0 (amniotic fluid peptide-1, human)
SB  - IM
MH  - Amino Acid Sequence
MH  - Amniotic Fluid/*chemistry
MH  - Anti-Infective Agents/*isolation & purification/*pharmacology
MH  - Female
MH  - Humans
MH  - Microbial Sensitivity Tests
MH  - Molecular Sequence Data
MH  - Peptide Fragments/*isolation & purification/*pharmacology
MH  - Pregnancy
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Ubiquitin/*isolation & purification/pharmacology
EDAT- 2007/08/03 09:00
MHDA- 2007/11/02 09:00
CRDT- 2007/08/03 09:00
PHST- 2007/05/12 00:00 [received]
PHST- 2007/06/28 00:00 [revised]
PHST- 2007/06/28 00:00 [accepted]
PHST- 2007/08/03 09:00 [pubmed]
PHST- 2007/11/02 09:00 [medline]
PHST- 2007/08/03 09:00 [entrez]
AID - S1570-9639(07)00151-3 [pii]
AID - 10.1016/j.bbapap.2007.06.013 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2007 Sep;1774(9):1221-6. doi: 10.1016/j.bbapap.2007.06.013. 
      Epub 2007 Jul 10.