PMID- 17670815
OWN - NLM
STAT- MEDLINE
DCOM- 20080108
LR  - 20220309
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 81
IP  - 20
DP  - 2007 Oct
TI  - Incorporation of high levels of chimeric human immunodeficiency virus envelope 
      glycoproteins into virus-like particles.
PG  - 10869-78
AB  - The human immunodeficiency virus (HIV) envelope (Env) protein is incorporated 
      into HIV virions or virus-like particles (VLPs) at very low levels compared to 
      the glycoproteins of most other enveloped viruses. To test factors that influence 
      HIV Env particle incorporation, we generated a series of chimeric gene constructs 
      in which the coding sequences for the signal peptide (SP), transmembrane (TM), 
      and cytoplasmic tail (CT) domains of HIV-1 Env were replaced with those of other 
      viral or cellular proteins individually or in combination. All constructs tested 
      were derived from HIV type 1 (HIV-1) Con-S DeltaCFI gp145, which itself was found 
      to be incorporated into VLPs much more efficiently than full-length Con-S Env. 
      Substitution of the SP from the honeybee protein mellitin resulted in 
      threefold-higher chimeric HIV-1 Env expression levels on insect cell surfaces and 
      an increase of Env incorporation into VLPs. Substitution of the HIV TM-CT with 
      sequences derived from the mouse mammary tumor virus (MMTV) envelope 
      glycoprotein, influenza virus hemagglutinin, or baculovirus (BV) gp64, but not 
      from Lassa fever virus glycoprotein, was found to enhance Env incorporation into 
      VLPs. The highest level of Env incorporation into VLPs was observed in chimeric 
      constructs containing the MMTV and BV gp64 TM-CT domains in which the Gag/Env 
      molar ratios were estimated to be 4:1 and 5:1, respectively, compared to a 56:1 
      ratio for full-length Con-S gp160. Electron microscopy revealed that VLPs with 
      chimeric HIV Env were similar to HIV-1 virions in morphology and size and 
      contained a prominent layer of Env spikes on their surfaces. HIV Env specific 
      monoclonal antibody binding results showed that chimeric Env-containing VLPs 
      retained conserved epitopes and underwent conformational changes upon CD4 
      binding.
FAU - Wang, Bao-Zhong
AU  - Wang BZ
AD  - Department of Microbiology and Immunology, Emory University School of Medicine, 
      1510 Clifton Road, Atlanta, GA 30322, USA.
FAU - Liu, Weimin
AU  - Liu W
FAU - Kang, Sang-Moo
AU  - Kang SM
FAU - Alam, Munir
AU  - Alam M
FAU - Huang, Chunzi
AU  - Huang C
FAU - Ye, Ling
AU  - Ye L
FAU - Sun, Yuliang
AU  - Sun Y
FAU - Li, Yingying
AU  - Li Y
FAU - Kothe, Denise L
AU  - Kothe DL
FAU - Pushko, Peter
AU  - Pushko P
FAU - Dokland, Terje
AU  - Dokland T
FAU - Haynes, Barton F
AU  - Haynes BF
FAU - Smith, Gale
AU  - Smith G
FAU - Hahn, Beatrice H
AU  - Hahn BH
FAU - Compans, Richard W
AU  - Compans RW
LA  - eng
GR  - P01 AI028147/AI/NIAID NIH HHS/United States
GR  - P30 AI027767/AI/NIAID NIH HHS/United States
GR  - P01 AI28147/AI/NIAID NIH HHS/United States
GR  - P30 AI27767/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20070801
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antigens, Viral)
RN  - 0 (CD4 Antigens)
RN  - 0 (Peptide Fragments)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (env Gene Products, Human Immunodeficiency Virus)
SB  - IM
MH  - Animals
MH  - Antigens, Viral
MH  - CD4 Antigens/metabolism
MH  - HIV/chemistry/ultrastructure
MH  - Humans
MH  - Peptide Fragments/genetics
MH  - Protein Structure, Tertiary
MH  - Recombinant Fusion Proteins/*metabolism
MH  - Recombination, Genetic
MH  - Virion/*growth & development/metabolism/ultrastructure
MH  - env Gene Products, Human Immunodeficiency Virus/*genetics/*metabolism
PMC - PMC2045522
EDAT- 2007/08/03 09:00
MHDA- 2008/01/09 09:00
PMCR- 2008/02/01
CRDT- 2007/08/03 09:00
PHST- 2007/08/03 09:00 [pubmed]
PHST- 2008/01/09 09:00 [medline]
PHST- 2007/08/03 09:00 [entrez]
PHST- 2008/02/01 00:00 [pmc-release]
AID - JVI.00542-07 [pii]
AID - 0542-07 [pii]
AID - 10.1128/JVI.00542-07 [doi]
PST - ppublish
SO  - J Virol. 2007 Oct;81(20):10869-78. doi: 10.1128/JVI.00542-07. Epub 2007 Aug 1.