PMID- 17671191
OWN - NLM
STAT- MEDLINE
DCOM- 20070911
LR  - 20131121
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 67
IP  - 15
DP  - 2007 Aug 1
TI  - Cannabinoid modulation of Kaposi's sarcoma-associated herpesvirus infection and 
      transformation.
PG  - 7230-7
AB  - Kaposi's sarcoma-associated herpesvirus (KSHV; also named human herpesvirus 8) is 
      necessary but not sufficient for the development of Kaposi's sarcoma. A variety 
      of factors may contribute to the pathogenesis of Kaposi's sarcoma in addition to 
      KSHV. Marijuana is a widely used recreational agent, and 
      Delta(9)-tetrahydrocannabinol (Delta(9)-THC), the major active component of 
      marijuana, is prescribed for medicinal use. To evaluate how cannabinoids may 
      affect the pathogenesis of Kaposi's sarcoma, we studied primary human dermal 
      microvascular endothelial cells (HMVEC) exposed to KSHV. There was an increased 
      efficiency of KSHV infection in the presence of low doses of Delta(9)-THC. We 
      also found that Delta(9)-THC increased the viral load in KSHV-infected HMVEC 
      through activation of the KSHV lytic switch gene, the open reading frame 50. 
      Furthermore, we observed that Delta(9)-THC stimulated expression of the 
      KSHV-encoded viral G protein-coupled receptor and Kaposi's sarcoma cell 
      proliferation. Our results indicate that Delta(9)-THC can enhance KSHV infection 
      and replication and foster KSHV-mediated endothelial transformation. Thus, use of 
      cannabinoids may place individuals at greater risk for the development and 
      progression of Kaposi's sarcoma.
FAU - Zhang, Xuefeng
AU  - Zhang X
AD  - Division of Experimental Medicine and Department of Surgery, Beth Israel 
      Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02115, 
      USA.
FAU - Wang, Jian Feng
AU  - Wang JF
FAU - Kunos, George
AU  - Kunos G
FAU - Groopman, Jerome E
AU  - Groopman JE
LA  - eng
GR  - 5R01HL061940/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (DNA, Viral)
RN  - 0 (G protein-coupled receptor, Human herpesvirus 8)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Rta protein, Human herpesvirus 8)
RN  - 0 (Trans-Activators)
RN  - 0 (Viral Proteins)
RN  - 16849-44-8 (delta(9-11)-tetrahydrocannabinol)
RN  - 7J8897W37S (Dronabinol)
SB  - IM
MH  - Blotting, Western
MH  - Cell Adhesion
MH  - Cell Proliferation/drug effects
MH  - Cell Transformation, Neoplastic/*drug effects
MH  - Cells, Cultured
MH  - DNA, Viral/analysis
MH  - Dronabinol/*analogs & derivatives/pharmacology
MH  - Endothelium, Vascular/drug effects/pathology/*virology
MH  - Herpesvirus 8, Human/drug effects/*physiology
MH  - Humans
MH  - Immediate-Early Proteins/metabolism
MH  - Receptors, Chemokine/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Skin/blood supply
MH  - Trans-Activators/metabolism
MH  - Transfection
MH  - Viral Load
MH  - Viral Proteins/metabolism
MH  - Virus Replication/drug effects
EDAT- 2007/08/03 09:00
MHDA- 2007/09/12 09:00
CRDT- 2007/08/03 09:00
PHST- 2007/08/03 09:00 [pubmed]
PHST- 2007/09/12 09:00 [medline]
PHST- 2007/08/03 09:00 [entrez]
AID - 67/15/7230 [pii]
AID - 10.1158/0008-5472.CAN-07-0960 [doi]
PST - ppublish
SO  - Cancer Res. 2007 Aug 1;67(15):7230-7. doi: 10.1158/0008-5472.CAN-07-0960.