PMID- 17671377
OWN - NLM
STAT- MEDLINE
DCOM- 20070910
LR  - 20181227
IS  - 1744-3091 (Electronic)
IS  - 1744-3091 (Linking)
VI  - 63
IP  - Pt 8
DP  - 2007 Aug 1
TI  - Molecular cloning, expression, purification and crystallographic analysis of 
      PRRSV 3CL protease.
PG  - 720-2
AB  - 3CL protease, a viral chymotrypsin-like proteolytic enzyme, plays a pivotal role 
      in the transcription and replication machinery of many RNA viruses, including 
      porcine reproductive and respiratory syndrome virus (PRRSV). In this study, the 
      full-length 3CL protease from PRRSV was cloned and overexpressed in Escherichia 
      coli. Crystallization experiments yielded crystals that diffracted to 2.1 A 
      resolution and belong to space group C2, with unit-cell parameters a = 112.31, b 
      = 48.34, c = 42.88 A, beta = 109.83 degrees . The Matthews coefficient and the 
      solvent content were calculated to be 2.49 A(3) Da(-1) and 50.61%, respectively, 
      for one molecule in the asymmetric unit.
FAU - Tian, Xinsheng
AU  - Tian X
AD  - Center for Molecular Immunology, Institute of Microbiology, Chinese Academy of 
      Sciences, Beijing 100101, People's Republic of China.
FAU - Feng, Youjun
AU  - Feng Y
FAU - Zhao, Tiezhu
AU  - Zhao T
FAU - Peng, Hao
AU  - Peng H
FAU - Yan, Jinghua
AU  - Yan J
FAU - Qi, Jianxun
AU  - Qi J
FAU - Jiang, Fan
AU  - Jiang F
FAU - Tian, Kegong
AU  - Tian K
FAU - Gao, Feng
AU  - Gao F
LA  - eng
SI  - PDB/1MBM
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070728
PL  - England
TA  - Acta Crystallogr Sect F Struct Biol Cryst Commun
JT  - Acta crystallographica. Section F, Structural biology and crystallization 
      communications
JID - 101226117
RN  - EC 3.4.- (Peptide Hydrolases)
SB  - IM
MH  - *Cloning, Molecular
MH  - Crystallography, X-Ray
MH  - Gene Expression Regulation, Enzymologic/*physiology
MH  - Peptide Hydrolases/biosynthesis/*chemistry/*genetics/isolation & purification
MH  - Porcine respiratory and reproductive syndrome virus/*enzymology/genetics
PMC - PMC2335157
EDAT- 2007/08/03 09:00
MHDA- 2007/09/11 09:00
PMCR- 2009/08/01
CRDT- 2007/08/03 09:00
PHST- 2007/05/09 00:00 [received]
PHST- 2007/07/07 00:00 [accepted]
PHST- 2007/08/03 09:00 [pubmed]
PHST- 2007/09/11 09:00 [medline]
PHST- 2007/08/03 09:00 [entrez]
PHST- 2009/08/01 00:00 [pmc-release]
AID - S1744309107033234 [pii]
AID - en5243 [pii]
AID - 10.1107/S1744309107033234 [doi]
PST - ppublish
SO  - Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Aug 1;63(Pt 8):720-2. doi: 
      10.1107/S1744309107033234. Epub 2007 Jul 28.