PMID- 17672248
OWN - NLM
STAT- MEDLINE
DCOM- 20070821
LR  - 20170214
IS  - 0003-4894 (Print)
IS  - 0003-4894 (Linking)
VI  - 116
IP  - 6
DP  - 2007 Jun
TI  - Quantitative analysis of OX62-positive dendritic cell distribution in the rat 
      laryngeal complex.
PG  - 449-56
AB  - OBJECTIVES: Dendritic cells (DCs) are key instigators of rejection after 
      transplantation. Their distribution has not been systematically characterized in 
      all locations of the larynx and its surrounding tissues. METHODS: Rat larynges 
      were stained with monoclonal antibodies identifying DCs. These cells were then 
      enumerated by a new combination of techniques including immunofluorescence, 
      confocal microscopy, and imaging software. RESULTS: The vast majority of DCs were 
      located in the epithelium and subepithelium of the airway; the mean DC density 
      ranged from 9 cells per square millimeter (0.2% of cells) to 645 cells per square 
      millimeter (10.3% of cells). Their density in the epithelium was 3 to 11 times 
      higher than that in the subepithelium. Non-airway sites (thyroid, parathyroid, 
      muscle, fat) had mean DC densities varying from 3 cells per square millimeter 
      (0.2%) to 57 cells per square millimeter (0.8%). No DCs were detected in 
      cartilage. CONCLUSIONS: Dendritic cells are concentrated in the laryngotracheal 
      epithelium and subepithelium and represent a much smaller proportion in the other 
      sites studied. A baseline for laryngeal DC population studies has been 
      established, and a computerized model for consistent quantitation using confocal 
      microscopy has been developed. This unique method will serve as a foundation for 
      investigating DC trafficking after rat laryngeal transplantation.
FAU - Friedman, Aaron D
AU  - Friedman AD
AD  - Head and Neck Institute, Cleveland Clinic, Cleveland, Ohio, USA.
FAU - Dan, Olivia
AU  - Dan O
FAU - Drazba, Judith A
AU  - Drazba JA
FAU - Lorenz, Robert R
AU  - Lorenz RR
FAU - Strome, Marshall
AU  - Strome M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Ann Otol Rhinol Laryngol
JT  - The Annals of otology, rhinology, and laryngology
JID - 0407300
RN  - 0 (Antigens, Differentiation)
RN  - 0 (OX-62 antigen, rat)
SB  - IM
MH  - Animals
MH  - Antigens, Differentiation/*metabolism
MH  - Dendritic Cells/*metabolism
MH  - Fluorescent Antibody Technique
MH  - Larynx/*cytology/*metabolism
MH  - Male
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Trachea/cytology/metabolism
EDAT- 2007/08/04 09:00
MHDA- 2007/08/22 09:00
CRDT- 2007/08/04 09:00
PHST- 2007/08/04 09:00 [pubmed]
PHST- 2007/08/22 09:00 [medline]
PHST- 2007/08/04 09:00 [entrez]
AID - 10.1177/000348940711600610 [doi]
PST - ppublish
SO  - Ann Otol Rhinol Laryngol. 2007 Jun;116(6):449-56. doi: 
      10.1177/000348940711600610.