PMID- 17674627
OWN - NLM
STAT- MEDLINE
DCOM- 20070828
LR  - 20191110
IS  - 1089-7771 (Print)
IS  - 1089-7771 (Linking)
VI  - 11
IP  - 4
DP  - 2007 Jul
TI  - Segmentation and classification of dot and non-dot-like fluorescence in situ 
      hybridization signals for automated detection of cytogenetic abnormalities.
PG  - 443-9
AB  - Signal segmentation and classification of fluorescence in situ hybridization 
      (FISH) images are essential for the detection of cytogenetic abnormalities. Since 
      current methods are limited to dot-like signal analysis, we propose a methodology 
      for segmentation and classification of dot and non-dot-like signals. First, 
      nuclei are segmented from their background and from each other in order to 
      associate signals with specific isolated nuclei. Second, subsignals composing 
      non-dot-like signals are detected and clustered to signals. Features are measured 
      to the signals and a subset of these features is selected representing the 
      signals to a multiclass classifier. Classification using a naive Bayesian 
      classifier (NBC) or a multilayer perceptron is accomplished. When applied to a 
      FISH image database, dot and non-dot-like signals were segmented almost perfectly 
      and then classified with accuracy of approximately 80% by either of the 
      classifiers.
FAU - Lerner, Boaz
AU  - Lerner B
AD  - Department of Electrical and Computer Engineering, Ben-Gurion University, Beer 
      Sheva 84105, Israel. boaz@ee.bgu.ac.il
FAU - Koushnir, Lev
AU  - Koushnir L
FAU - Yeshaya, Josepha
AU  - Yeshaya J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - IEEE Trans Inf Technol Biomed
JT  - IEEE transactions on information technology in biomedicine : a publication of the 
      IEEE Engineering in Medicine and Biology Society
JID - 9712259
SB  - IM
MH  - Algorithms
MH  - *Artificial Intelligence
MH  - Cell Nucleus/*genetics
MH  - *Chromosome Aberrations
MH  - Chromosome Mapping/methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Pattern Recognition, Automated/*methods
MH  - Sequence Analysis, DNA/*methods
EDAT- 2007/08/07 09:00
MHDA- 2007/08/29 09:00
CRDT- 2007/08/07 09:00
PHST- 2007/08/07 09:00 [pubmed]
PHST- 2007/08/29 09:00 [medline]
PHST- 2007/08/07 09:00 [entrez]
AID - 10.1109/titb.2007.894335 [doi]
PST - ppublish
SO  - IEEE Trans Inf Technol Biomed. 2007 Jul;11(4):443-9. doi: 
      10.1109/titb.2007.894335.