PMID- 17675272
OWN - NLM
STAT- MEDLINE
DCOM- 20071127
LR  - 20191210
IS  - 0925-5710 (Print)
IS  - 0925-5710 (Linking)
VI  - 86
IP  - 1
DP  - 2007 Jul
TI  - Human leukocyte antigens A and B Loci genotyping by reference strand-mediated 
      conformation analysis in hematopoietic stem cell transplantation donor selection.
PG  - 77-83
AB  - Research has been carried out to evaluate the reference strand-mediated 
      conformation analysis (RSCA) typing method on human leukocyte antigen (HLA)-A and 
      -B loci matching in donor selection for hematopoietic stem cell transplantation 
      (HSCT). Twenty standard DNA samples from the RSCA program of the 13th 
      International Histocompatibiliy Working Group, and 124 DNA samples extracted from 
      peripheral blood cells of 39 patients and 85 related potential donors were typed 
      both by RSCA and polymerase chain reaction sequence-specific primer (PCR-SSP) for 
      their HLA-A and -B genes. The ambiguous results were further confirmed by 
      sequence-based typing (SBT). HLA-A and -B genotypes assigned by RSCA correlated 
      well with PCR-SSP and the repetitive rate of RSCA was 100%. In our study, 33/84 
      (39%) and 67/90 (72.8%) RSCA genotyping results of HLA-A and -B loci could be 
      typed to allelic level, respectively. RSCA can detect mismatches that are not 
      routinely identified by the PCR-SSP method and can detect the chimerism status 
      after patients have gone through HLA-mismatched HSCT. Eight ambiguous samples 
      were confirmed by SBT and the results indicated that RSCA was more accurate than 
      low-resolution PCR-SSP and its database should be improved. RSCA is reproducible, 
      has a high resolution, is able to detect chimerism follow-up after HLA-mismatched 
      HSCT, and is a useful approach for donor selection with some insufficiencies to 
      be improved.
FAU - Sun, Yuying
AU  - Sun Y
AD  - Department of Immunology and National Center for Biomedicine Analysis, Beijing 
      307 Hospital Affiliated with the Academy of Medical Sciences, Beijing, China.
FAU - Zhao, Dandan
AU  - Zhao D
FAU - Jin, Li
AU  - Jin L
FAU - Liu, Nan
AU  - Liu N
FAU - Liang, Fei
AU  - Liang F
FAU - Kong, Fanhua
AU  - Kong F
FAU - Xi, Yongzhi
AU  - Xi Y
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Int J Hematol
JT  - International journal of hematology
JID - 9111627
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Base Sequence/genetics
MH  - DNA/analysis
MH  - Genotype
MH  - HLA-A Antigens/*genetics
MH  - HLA-B Antigens/*genetics
MH  - Hematopoietic Stem Cell Transplantation/methods
MH  - Histocompatibility Testing/*methods
MH  - Humans
MH  - *Polymorphism, Single-Stranded Conformational
MH  - Tissue Donors/*classification
MH  - Transplantation, Homologous/methods
EDAT- 2007/08/07 09:00
MHDA- 2007/12/06 09:00
CRDT- 2007/08/07 09:00
PHST- 2007/08/07 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/08/07 09:00 [entrez]
AID - K72M167388M074V4 [pii]
AID - 10.1532/IJH97.A10611 [doi]
PST - ppublish
SO  - Int J Hematol. 2007 Jul;86(1):77-83. doi: 10.1532/IJH97.A10611.