PMID- 17675476
OWN - NLM
STAT- MEDLINE
DCOM- 20071003
LR  - 20190516
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 179
IP  - 4
DP  - 2007 Aug 15
TI  - Selective expansion of foxp3-positive regulatory T cells and immunosuppression by 
      suppressors of cytokine signaling 3-deficient dendritic cells.
PG  - 2170-9
AB  - Dendritic cells (DCs) induce immunity and immunological tolerance as APCs. It has 
      been shown that DCs secreting IL-10 induce IL-10(+) Tr1-type regulatory T (Treg) 
      cells, whereas Foxp3-positive Treg cells are expanded from naive CD4(+) T cells 
      by coculturing with mature DCs. However, the regulatory mechanism of expansion of 
      Foxp3(+) Treg cells by DCs has not been clarified. In this study, we demonstrated 
      that suppressors of cytokine signaling (SOCS)-3-deficient DCs have a strong 
      potential as Foxp3(+) T cell-inducing tolerogenic DCs. SOCS3(-/-) DCs expressed 
      lower levels of class II MHC, CD40, CD86, and IL-12 than wild-type (WT)-DCs both 
      in vitro and in vivo, and showed constitutive activation of STAT3. Foxp3(-) 
      effector T cells were predominantly expanded by the priming with WT-DCs, whereas 
      Foxp3(+) Treg cells were selectively expanded by SOCS3(-/-) DCs. Adoptive 
      transfer of SOCS3(-/-) DCs reduced the severity of experimental autoimmune 
      encephalomyelitis. Foxp3(+) T cell expansion was blocked by anti-TGF-beta Ab, and 
      SOCS3(-/-) DCs produced higher levels of TGF-beta than WT-DCs, suggesting that 
      TGF-beta plays an essential role in the expansion of Foxp3(+) Treg cells. These 
      results indicate an important role of SOCS3 in determining on immunity or 
      tolerance by DCs.
FAU - Matsumura, Yumiko
AU  - Matsumura Y
AD  - Division of Molecular and Cellular Immunology, Medical Institute of 
      Bioregulation, Kyushu University, Fukuoka, Japan.
FAU - Kobayashi, Takashi
AU  - Kobayashi T
FAU - Ichiyama, Kenji
AU  - Ichiyama K
FAU - Yoshida, Ryoko
AU  - Yoshida R
FAU - Hashimoto, Masayuki
AU  - Hashimoto M
FAU - Takimoto, Tomohito
AU  - Takimoto T
FAU - Tanaka, Kentaro
AU  - Tanaka K
FAU - Chinen, Takatoshi
AU  - Chinen T
FAU - Shichita, Takashi
AU  - Shichita T
FAU - Wyss-Coray, Tony
AU  - Wyss-Coray T
FAU - Sato, Katsuaki
AU  - Sato K
FAU - Yoshimura, Akihiko
AU  - Yoshimura A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antibodies)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD40 Antigens)
RN  - 0 (Cd86 protein, mouse)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Foxp3 protein, mouse)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Socs3 protein, mouse)
RN  - 0 (Suppressor of Cytokine Signaling 3 Protein)
RN  - 0 (Suppressor of Cytokine Signaling Proteins)
RN  - 0 (Transforming Growth Factor beta)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Adoptive Transfer
MH  - Animals
MH  - Antibodies/immunology/pharmacology
MH  - B7-2 Antigen/genetics/immunology
MH  - CD40 Antigens/genetics/immunology
MH  - *Cell Proliferation
MH  - Coculture Techniques
MH  - Dendritic Cells/*immunology
MH  - Forkhead Transcription Factors/genetics/*immunology
MH  - Histocompatibility Antigens Class II/genetics/immunology
MH  - Immune Tolerance/drug effects/genetics/*immunology
MH  - Interleukin-10/genetics/immunology
MH  - Interleukin-12/genetics/immunology
MH  - Mice
MH  - Mice, Knockout
MH  - Suppressor of Cytokine Signaling 3 Protein
MH  - Suppressor of Cytokine Signaling Proteins/deficiency/*immunology
MH  - T-Lymphocytes, Regulatory/*immunology
MH  - Transforming Growth Factor beta/genetics/immunology
EDAT- 2007/08/07 09:00
MHDA- 2007/10/04 09:00
CRDT- 2007/08/07 09:00
PHST- 2007/08/07 09:00 [pubmed]
PHST- 2007/10/04 09:00 [medline]
PHST- 2007/08/07 09:00 [entrez]
AID - 179/4/2170 [pii]
AID - 10.4049/jimmunol.179.4.2170 [doi]
PST - ppublish
SO  - J Immunol. 2007 Aug 15;179(4):2170-9. doi: 10.4049/jimmunol.179.4.2170.