PMID- 17675862
OWN - NLM
STAT- MEDLINE
DCOM- 20070820
LR  - 20070806
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 117
IP  - 1-4
DP  - 2007
TI  - Characterization of the turkey MHC chromosome through genetic and physical 
      mapping.
PG  - 213-20
AB  - Previous studies in the chicken have identified a single microchromosome (GGA16) 
      containing the ribosomal DNA (rDNA) and two genetically unlinked MHC regions, 
      MHC-B and MHC-Y. Chicken DNA sequence from these loci was used to develop PCR 
      primers for amplification of homologous fragments from the turkey (Meleagris 
      gallopavo). PCR products were sequenced and overgo probes were designed to screen 
      the CHORI 260 turkey BAC library. BAC clones corresponding to the turkey rDNA, 
      MHC-B and MHC-Y were identified. BAC end and subclone sequencing confirmed 
      identity and homology of the turkey BAC clones to the respective chicken loci. 
      Based on subclone sequences, single-nucleotide polymorphisms (SNPs) segregating 
      within the UMN/NTBF mapping population were identified and genotyped. Analysis of 
      SNP genotypes found the B and Y to be genetically unlinked in the turkey. Silver 
      staining of metaphase chromosomes identified a single pair of microchromosomes 
      with nucleolar organizer regions (NORs). Physical locations of the rDNA and MHC 
      loci were determined by fluorescence in situ hybridization (FISH) of the BAC 
      clones to metaphase chromosomes. FISH clearly positioned the rDNA distal to the Y 
      locus on the q-arm of the MHC chromosome and the MHC-B on the p-arm. An internal 
      telomere array on the MHC chromosome separates the B and Y loci.
CI  - Copyright 2007 S. Karger AG, Basel.
FAU - Chaves, L D
AU  - Chaves LD
AD  - Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, 
      University of Minnesota, St. Paul, MN 55108, USA.
FAU - Krueth, S B
AU  - Krueth SB
FAU - Reed, K M
AU  - Reed KM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (Histocompatibility Antigens)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Chromosomes/*genetics/*immunology
MH  - Cloning, Molecular
MH  - Databases, Nucleic Acid
MH  - Genomics
MH  - Histocompatibility Antigens/*genetics/*immunology
MH  - In Situ Hybridization
MH  - Metaphase
MH  - Nucleic Acid Amplification Techniques
MH  - Physical Chromosome Mapping
MH  - Turkeys/*genetics/*immunology
EDAT- 2007/08/07 09:00
MHDA- 2007/08/21 09:00
CRDT- 2007/08/07 09:00
PHST- 2006/07/07 00:00 [received]
PHST- 2006/09/07 00:00 [accepted]
PHST- 2007/08/07 09:00 [pubmed]
PHST- 2007/08/21 09:00 [medline]
PHST- 2007/08/07 09:00 [entrez]
AID - 000103182 [pii]
AID - 10.1159/000103182 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2007;117(1-4):213-20. doi: 10.1159/000103182.