PMID- 17678915
OWN - NLM
STAT- MEDLINE
DCOM- 20080310
LR  - 20080215
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Linking)
VI  - 89
IP  - 2
DP  - 2008 Feb
TI  - Endometrial and peritoneal expression of aromatase, cytokines, and adhesion 
      factors in women with endometriosis.
PG  - 301-10
AB  - OBJECTIVE: To examine messenger (m) RNA expression of aromatase, cytokines, and 
      adhesion factors in women with and without endometriosis. DESIGN: Patients with 
      endometriosis were compared with control patients. SETTING: University Hospital 
      Gasthuisberg, Leuven, Belgium. PATIENT(S): A total of 35 patients who had 
      laparoscopic surgery during the luteal phase (n = 20) or the menstrual phase (n = 
      15) were selected for this study based on cycle phase and presence/absence of 
      endometriosis. INTERVENTION(S): Tissues of endometrium and macroscopically normal 
      peritoneum were collected during hysteroscopy and laparoscopic surgery, 
      respectively, from 24 women with revised American Society for Reproductive 
      Medicine stage (rASRM) stages I-II (n = 12) and III-IV (n = 12) endometriosis and 
      11 control patients with normal pelvic. Tissue samples were selected from a 
      tissue bank, based on the phase of the cycle (menstrual or luteal) and the 
      presence/absence of endometriosis. MAIN OUTCOME MEASURE(S): The mRNA levels of 
      aromatase, vimentin, vascular cell adhesion molecule 1 (VCAM-1), alpha(V) and 
      beta(3) integrins, interleukin (IL)-1 beta, regulated on activation normal T-cell 
      expressed and secreted (RANTES), and monocyte chemotactic protein 1 (MCP-1) were 
      evaluated using real-time reverse transcriptase polymerase chain reaction. 
      RESULT(S): During menstrual phase, increased endometrial mRNA levels of alpha(V) 
      integrin, combined alpha(V)beta(3) integrins, and increased peritoneal IL-1 beta 
      mRNA levels--but decreased peritoneal MCP-1 mRNA levels--were observed in women 
      with endometriosis compared with control subjects. During luteal phase, 
      endometrial mRNA levels of IL-1 beta and RANTES were increased in women with 
      endometriosis compared with control subjects. Endometrial aromatase mRNA 
      expression was higher in women with endometriosis than in control subjects in 
      combined phases. Women with endometriosis had increased peritoneal mRNA 
      expression of RANTES and VCAM-1 during menstrual compared with luteal phase. 
      CONCLUSION(S): Aberrant mRNA expression of aromatase, cytokines, and adhesion 
      factors in endometrium and peritoneum suggests that both tissues are involved in 
      the pathogenesis of endometriosis.
FAU - Kyama, Cleophas M
AU  - Kyama CM
AD  - Leuven University Fertility Center, Department of Obstetrics and Gynecology, 
      University Hospital Gasthuisberg, Leuven, Belgium.
FAU - Overbergh, Lutgart
AU  - Overbergh L
FAU - Mihalyi, Attila
AU  - Mihalyi A
FAU - Meuleman, Christel
AU  - Meuleman C
FAU - Mwenda, Jason M
AU  - Mwenda JM
FAU - Mathieu, Chantal
AU  - Mathieu C
FAU - D'Hooghe, Thomas M
AU  - D'Hooghe TM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070806
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Chemokine CCL5)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1beta)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 0 (Vimentin)
RN  - EC 1.14.14.1 (Aromatase)
SB  - IM
MH  - Adult
MH  - Aromatase/*genetics/metabolism
MH  - Cell Adhesion Molecules/*genetics/metabolism
MH  - Chemokine CCL5/genetics/metabolism
MH  - Cytokines/*genetics/metabolism
MH  - Endometriosis/*genetics/metabolism
MH  - Endometrium/*metabolism
MH  - Female
MH  - Humans
MH  - Interleukin-1beta/genetics/metabolism
MH  - Luteal Phase/genetics/metabolism
MH  - Menstruation/genetics/metabolism
MH  - Peritoneum/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Receptors, CCR2/genetics/metabolism
MH  - Uterine Diseases/*genetics/metabolism
MH  - Vascular Cell Adhesion Molecule-1/genetics/metabolism
MH  - Vimentin/genetics/metabolism
EDAT- 2007/08/07 09:00
MHDA- 2008/03/11 09:00
CRDT- 2007/08/07 09:00
PHST- 2006/11/17 00:00 [received]
PHST- 2007/02/27 00:00 [revised]
PHST- 2007/02/27 00:00 [accepted]
PHST- 2007/08/07 09:00 [pubmed]
PHST- 2008/03/11 09:00 [medline]
PHST- 2007/08/07 09:00 [entrez]
AID - S0015-0282(07)00525-0 [pii]
AID - 10.1016/j.fertnstert.2007.02.057 [doi]
PST - ppublish
SO  - Fertil Steril. 2008 Feb;89(2):301-10. doi: 10.1016/j.fertnstert.2007.02.057. Epub 
      2007 Aug 6.