PMID- 17679364
OWN - NLM
STAT- MEDLINE
DCOM- 20071126
LR  - 20130520
IS  - 1426-9686 (Print)
IS  - 1426-9686 (Linking)
VI  - 22
IP  - 131
DP  - 2007 May
TI  - [The participation of some innate immunological defense components in children 
      with chronic gastritis associated H. pylori infection in ultrastructure and 
      function of the mast cells aspects].
PG  - 346-9
AB  - It has been clearly established that Helicobacter pyloni (H. pylori) play an 
      important role in the pathogenesis of some chronic diseases of upper 
      gastrointestinal tract. A lot of attention to the complicated immunological 
      processes induced by the infection was paid. The clinical outcome of the damage 
      of gastric mucosa by H. pylori depends on the type and the intensification of 
      these processes. During many years the acquired (specific) immunological response 
      on the infection was analyzed by scientists, but much more researches on innate 
      defense was done lately The mast cells constitute some important parts of the 
      immunological innate defense. H. pylori colonization of gastric mucosal surface 
      elicits a conspicuous infiltration comprising of lymphocytes, and plasma cells as 
      well as neutrophils, eosinophils, macrophages and the granule cells of connective 
      fissue. Some difficulties with the mastocytes visualization in conventional 
      histological slides in light microscope caused so far the little attention of 
      their participation in chronic gastritis. The aim of the study was the assessment 
      of the mastocytes participation in the infiltration of immunological cells 
      induced by H. pylonri in chronic gastritis. MATERIAL AND METHODS: The subjects 
      were twenty dyspeptic children aged 9-17 years underwent upper GI endoscopy 
      procedure. Twelve children were H. pylori positive. Remaining eight children H. 
      pylori negative composed a control group. Gastric antrum and corpus tissue 
      specimens in the conventional (light) and electron microscopy were examined. The 
      specimens intended for electron microscope assessment were fixed in the solution 
      of 1% glutaraldehyde and 2.5% paraformaldehyde at 40 C (pH 7.4) for 24 hours, and 
      postfixed in 2% osmium tetroxide at the same conditions. Ultrathin sections were 
      contrasted with uranyl acetate and lead citrate. Results. Ultrastructural 
      analysis revealed two distinct, morphological forms of mast cells in gastric 
      mucosa. All mast cells contained multiple granules with fine-grained material but 
      their appearance was distinct in both types of these cells. Statistical analysis 
      revealed that the count of mast cells in gastric mucosa was increased in H. 
      pylori positive when compared with H. pylori negative children. It was also 
      affirmed that in specimens from H. pylori infected children mast cells more 
      frequently were seen in the gastric epithelium.
FAU - Czkwianianc, Elzbieta
AU  - Czkwianianc E
AD  - Klinik Gastroenterologii, Instytutu Centrum Zdrowia Matki Polki w Lodzi, 
      Uniwersytet Medyczny w Lodzi. kpg@iczmp.edu.pl
FAU - Janas, Bogdan
AU  - Janas B
FAU - Bartel, Hieronim
AU  - Bartel H
FAU - Ryszard, Makosiej
AU  - Ryszard M
FAU - Durko, Alina
AU  - Durko A
FAU - Malecka-Panas, Ewa
AU  - Malecka-Panas E
LA  - pol
PT  - English Abstract
PT  - Journal Article
TT  - Udzial niektorych elementow odpornosci wrodzonej w zapaleniu blony sluzowej 
      zoladka z zakaieniem H. pylori u dzieci w aspekcie ultrastruktury i funkcji 
      komorki tucznej.
PL  - Poland
TA  - Pol Merkur Lekarski
JT  - Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego
JID - 9705469
SB  - IM
MH  - Adolescent
MH  - Biopsy, Needle
MH  - Child
MH  - Chronic Disease
MH  - Gastric Mucosa/*immunology/ultrastructure
MH  - Gastritis/*immunology/microbiology/pathology
MH  - Helicobacter Infections/*immunology/microbiology/pathology
MH  - Humans
MH  - Immunity, Innate/physiology
MH  - Mast Cells/*immunology/*ultrastructure
MH  - Microscopy, Electron
MH  - Pyloric Antrum/*immunology/ultrastructure
EDAT- 2007/08/08 09:00
MHDA- 2007/12/06 09:00
CRDT- 2007/08/08 09:00
PHST- 2007/08/08 09:00 [pubmed]
PHST- 2007/12/06 09:00 [medline]
PHST- 2007/08/08 09:00 [entrez]
PST - ppublish
SO  - Pol Merkur Lekarski. 2007 May;22(131):346-9.