PMID- 17679422
OWN - NLM
STAT- MEDLINE
DCOM- 20100610
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 25
IP  - 3
DP  - 2007 May
TI  - [On-line sample stacking for determination of carnosine-related peptides by 
      capillary electrophoresis].
PG  - 326-31
AB  - Two on-line sample stacking methods in capillary electrophoresis, large volume 
      sample stacking using reversed pressure as a pump (LVSRP) and large volume sample 
      stacking using electroosmotic flow (EOF) pump (LVSEP), were developed for the 
      separation and determination of carnosine, anserine and homocarnosine. The 
      principles of the two stacking methods are described. Various parameters 
      affecting capillary electrophoresis separation and sample stacking were studied 
      and optimized. The optimal stacking conditions for LVSRP were as follows: 
      hydrodynamic sample injection at 41.4 kPa (60 s), then injection of a short plug 
      of buffer at 13.8 kPa x 15 s, stacking of sample at a voltage of 10 kV with a 
      back pressure of 34.5 kPa, and separation in phosphate buffer (pH 5.63, 100 
      mmol/L) at a voltage of 15 kV. The optimal stacking conditions for LVSEP were as 
      follows: hydrodynamic sample injection at 48.3 kPa (60 s), stacking at a voltage 
      of 20 kV and separation at a voltage of - 20 kV in a buffer of 100 mmol/L 
      phosphate-0.5 mmol/L cetyltrimethylammonium bromide (CTAB) (pH 5.31). About 40 - 
      60 fold improvement of sensitivity was achieved without loss of separation 
      efficiency by LVSRP and LVSEP, when compared with the normal CZE method. Under 
      the optimum conditions, excellent linear responses were obtained in the 
      concentration range of 0.080 - 5.0 micromol/L. The relative standard deviations 
      (RSDs) of peak height were 2.4% - 2.7% and 0.6% - 4.0% for LVSRP and LVSEP, 
      respectively. The concentration limits of detection (defined as signal-to-noise 
      ratio of 3) of carnosine, anserine and homocarnosine obtained for LVSRP and LVSEP 
      were 41 - 58 nmol/L and 35 - 43 nmol/L, respectively.
FAU - Huang, Ying
AU  - Huang Y
AD  - College of Chemistry and Materials Science, Fujian Normal University, Fuzhou 
      350007, China. huang-ying@fzu.edu.cn
FAU - Duan, Jianping
AU  - Duan J
FAU - Zhang, Jianhua
AU  - Zhang J
FAU - Chen, Guonan
AU  - Chen G
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Peptides)
RN  - 8HO6PVN24W (Carnosine)
SB  - IM
MH  - Carnosine/*chemistry
MH  - Electrophoresis, Capillary/*methods
MH  - Limit of Detection
MH  - Peptides/*chemistry
EDAT- 2007/08/08 09:00
MHDA- 2010/06/11 06:00
CRDT- 2007/08/08 09:00
PHST- 2007/08/08 09:00 [pubmed]
PHST- 2010/06/11 06:00 [medline]
PHST- 2007/08/08 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2007 May;25(3):326-31.