PMID- 17679933
OWN - NLM
STAT- MEDLINE
DCOM- 20070918
LR  - 20181201
IS  - 1090-0535 (Electronic)
IS  - 1090-0535 (Linking)
VI  - 13
DP  - 2007 Jul 25
TI  - Comparison of expression profile of neurotrophins and their receptors in primary 
      and transformed rat retinal ganglion cells.
PG  - 1311-8
AB  - PURPOSE: To determine the expression profile of neurotrophins and their receptors 
      in cultured primary rat retinal ganglion cells (RGCs) and the transformed RGC-5 
      cells. METHODS: Confocal microscopic immunocytochemistry with double fluorescent 
      labeling with thy-1 as a marker for RGCs was used to demonstrate expression of 
      neurotrophins and their receptors. An enzyme-linked immunosorbent assay (ELISA) 
      was used to detect secretion of neurotrophins by RGC-5 cells. RESULTS: Primary 
      RGCs and RGC-5 cells expressed brain-derived neurotrophic factor (BDNF), nerve 
      growth factor (NGF), neurotrophin-3 (NT3), neurotrophin-4 (NT4), and receptors 
      TrkA, p75, with low levels of TrkB. However, minimal if any, expression of TrkC 
      was observed in these cells. RGC-5 cells also secreted NT3 (1311+/-21 pg/ml), 
      BDNF (92+/-9 pg/ml), NGF (86+/-7 pg/ml), and NT4 (21+/-1 pg/ml) into the cultured 
      media. CONCLUSIONS: These results demonstrated that neurotrophins and TrkA, p75 
      with low levels of TrkB receptors are expressed by RGCs. Specific neurotrophins 
      acting through TrkA, TrkB, and p75 receptors within the RGCs may be involved in 
      the survival and apoptosis of the RGCs in various retinopathies, such as 
      glaucoma.
FAU - Agarwal, Neeraj
AU  - Agarwal N
AD  - Department of Cell Biology and Genetics, UNT Health Science Center, Fort Worth, 
      TX 76107, USA. nagarwal@hsc.unt.edu
FAU - Agarwal, Rajnee
AU  - Agarwal R
FAU - Kumar, Domalapalli Maneesh
AU  - Kumar DM
FAU - Ondricek, Amber
AU  - Ondricek A
FAU - Clark, Abbot F
AU  - Clark AF
FAU - Wordinger, Robert J
AU  - Wordinger RJ
FAU - Pang, Iok-Hou
AU  - Pang IH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20070725
PL  - United States
TA  - Mol Vis
JT  - Molecular vision
JID - 9605351
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 0 (Thy-1 Antigens)
SB  - IM
MH  - Animals
MH  - Cell Line, Transformed
MH  - *Gene Expression Profiling
MH  - Immunohistochemistry
MH  - Nerve Growth Factors/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Nerve Growth Factor/*metabolism
MH  - Retinal Ganglion Cells/cytology/*metabolism
MH  - Thy-1 Antigens/metabolism
EDAT- 2007/08/08 09:00
MHDA- 2007/09/19 09:00
CRDT- 2007/08/08 09:00
PHST- 2007/08/08 09:00 [pubmed]
PHST- 2007/09/19 09:00 [medline]
PHST- 2007/08/08 09:00 [entrez]
AID - v13/a143 [pii]
PST - epublish
SO  - Mol Vis. 2007 Jul 25;13:1311-8.