PMID- 17680522
OWN - NLM
STAT- MEDLINE
DCOM- 20090327
LR  - 20070807
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 24
IP  - 4
DP  - 2007 Aug
TI  - [Clinical and experimental retrospective analysis on acute leukemia with trisomy 
      4 cell].
PG  - 369-72
AB  - OBJECTIVE: To explore the clinical and experimental features of acute leukemia 
      (AL) with trisomy 4. METHODS: A retrospective analysis on the clinical and 
      laboratory data of 21 cases of AL with trisomy 4 was performed. Chromosomes were 
      prepared using direct method and/or short-term (24 h) cultures of bone marrow 
      cells. Karyotypic analysis was carried out by using R-banding technique. Thirteen 
      cases were studied by interphase fluorescence in situ hybridization (FISH) by 
      using a chromosome 4-specific alpha -satellite DNA probe labeled by spectrum 
      Green to ascertain the presence of a clone with trisomy 4. Five cases with t (8; 
      21) revealed by karyotypic analysis were detected by dual-color FISH using t (8; 
      21) translocation probe to confirm the AML1/ETO rearrangement. RESULTS: All the 
      patients with AL and trisomy 4 were with de novo AL except two cases with 
      secondary AL. M2 was the most frequent Franch-American-British(FAB) subtype in 
      this series (9/21 cases). The initial leukocyte count more than 10x 10(9)/L was 
      seen in 16 cases. An enlargement of liver, spleen and/or lymph nodes in varying 
      degrees was found in 15 cases. Among 15 cases received immunophenotypic analysis, 
      11 cases showed CD34 positivity and 6 cases co-expressed myeloid and lymphocyte 
      antigens. Karyotypic analysis disclosed clonal trisomy 4 in 18 cases and one cell 
      with +4 in 3 cases. Isolated trisomy 4 was found in 7 cases, while 14 cases had 
      other abnormalities besides trisomy 4 among which t (8; 21) was found in 8 cases. 
      Dual-color FISH confirmed that all 13 cases including 3 cases having one cell 
      with +4 on karyotypic analysis had clonal trisomy 4. Dual-color FISH confirmed 
      that all 5 cases with t (8; 21) had AML1/ETO rearrangement. CONCLUSION: AL 
      patients with trisomy 4 have unique clinical and experimental features and a poor 
      prognosis.
FAU - Pan, Jin-lan
AU  - Pan JL
AD  - The First Affiliated Hospital, Soochow University, Jiangsu Institute of 
      Hematology, Suzhou, Jiangsu, 215006 P. R. China.
FAU - Xue, Yong-quan
AU  - Xue YQ
FAU - Qiu, Hui-ying
AU  - Qiu HY
FAU - Zhang, Jun
AU  - Zhang J
FAU - Wu, Ya-fang
AU  - Wu YF
FAU - Wang, Yong
AU  - Wang Y
FAU - Shen, Juan
AU  - Shen J
FAU - Zhu, Yong-jin
AU  - Zhu YJ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - Acute Disease
MH  - Adult
MH  - Aged
MH  - Chromosomes, Human, Pair 4/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping/methods
MH  - Leukemia/*genetics
MH  - Male
MH  - Middle Aged
MH  - Trisomy/*genetics
MH  - Young Adult
EDAT- 2007/08/08 09:00
MHDA- 2009/03/28 09:00
CRDT- 2007/08/08 09:00
PHST- 2007/08/08 09:00 [pubmed]
PHST- 2009/03/28 09:00 [medline]
PHST- 2007/08/08 09:00 [entrez]
AID - 940624083 [pii]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2007 Aug;24(4):369-72.