PMID- 17680526
OWN - NLM
STAT- MEDLINE
DCOM- 20090327
LR  - 20211203
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 24
IP  - 4
DP  - 2007 Aug
TI  - [The relationship of mTOR signaling pathway and histone acetylation in human 
      gastric cancer cell lines].
PG  - 387-91
AB  - OBJECTIVE: To evaluate the relationship between mammalian target of rapamycin 
      (mTOR) signaling pathway and histone acetylation in cell survival, cell cycle, 
      gene expression and protein level on human gastric cancer cells. METHODS: Human 
      gastric cancer cell lines, MKN45 and SGC7901 were treated with trichostatin A, 
      rapamycin and/or LY294002, a PI3K inhibitor. Cell viability was analyzed by 
      methylthiazolyl tetrazolium. Cell cycle distribution was evaluated by flow 
      cytometry. The transcription level of p21(WAF1) gene was detected by using 
      real-time polymerase chain reaction. Proteins were detected by Western blotting. 
      RESULTS: Cell viability remarkably reduced after treatment by more than two drugs 
      (P< 0.01). Through flow cytometry assessment, MKN45 cells were arrested in G2 
      phase (P< 0.05), while SGC7901 cells were in G2 or G1 phase (P< 0.05) whether 
      treated with single or more than two drugs. The expression of p21(WAF1) mRNA was 
      remarkably increased in the gastric cancer cells treated with conjoined drugs (P< 
      0.01). Phosphorylation of Akt, p70S6K and 4E-BP1 was significantly reduced in 
      cells treated with conjoined drugs (P< 0.01). And histone acetylation of H4/H3 
      was also increased in cells treated with conjoined drugs (P< 0.01). CONCLUSION: 
      mTOR singnaling pathway has an important relationship with histone acetylation in 
      gastric cancer cell lines. There is a co-effect of mTOR inhibitor and histone 
      deacetylase inhibitor on gastric cancer cells.
FAU - Sun, Dan-feng
AU  - Sun DF
AD  - Renji Hospital, Shanghai Institute of Digestive Disease, School of Medicine, 
      Shanghai Jiaotong University, Shanghai, 200001 P. R. China.
FAU - Fang, Jing-yuan
AU  - Fang JY
FAU - Zhang, Yan-jie
AU  - Zhang YJ
FAU - Tian, Xiao-qing
AU  - Tian XQ
FAU - Zhu, Hong-yin
AU  - Zhu HY
FAU - Li, En-ling
AU  - Li EL
FAU - Gu, Wei-qi
AU  - Gu WQ
FAU - Shen, Guan-feng
AU  - Shen GF
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (CDKN1A protein, human)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Chromones)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p21)
RN  - 0 (EIF4EBP1 protein, human)
RN  - 0 (Histones)
RN  - 0 (Hydroxamic Acids)
RN  - 0 (Morpholines)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one)
RN  - 3X2S926L3Z (trichostatin A)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Acetylation/drug effects
MH  - Adaptor Proteins, Signal Transducing/metabolism
MH  - Blotting, Western
MH  - Cell Cycle/drug effects
MH  - Cell Cycle Proteins
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Chromones/pharmacology
MH  - Cyclin-Dependent Kinase Inhibitor p21/genetics
MH  - Flow Cytometry
MH  - Histones/*metabolism
MH  - Humans
MH  - Hydroxamic Acids/pharmacology
MH  - Morpholines/pharmacology
MH  - Phosphoproteins/metabolism
MH  - Phosphorylation/drug effects
MH  - Polymerase Chain Reaction
MH  - Protein Kinases/*metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Ribosomal Protein S6 Kinases, 70-kDa/metabolism
MH  - Signal Transduction/drug effects/*physiology
MH  - Sirolimus/pharmacology
MH  - Stomach Neoplasms/metabolism/pathology/physiopathology
MH  - TOR Serine-Threonine Kinases
EDAT- 2007/08/08 09:00
MHDA- 2009/03/28 09:00
CRDT- 2007/08/08 09:00
PHST- 2007/08/08 09:00 [pubmed]
PHST- 2009/03/28 09:00 [medline]
PHST- 2007/08/08 09:00 [entrez]
AID - 940624087 [pii]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2007 Aug;24(4):387-91.