PMID- 17680547
OWN - NLM
STAT- MEDLINE
DCOM- 20090327
LR  - 20070807
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 24
IP  - 4
DP  - 2007 Aug
TI  - [Molecular genetics in chronic myeloid leukemia with variant Ph translocation].
PG  - 470-3
AB  - OBJECTIVE: To explore the value of fluorescence in situ hybridization (FISH) and 
      multiplex fluorescence in situ hybridization (M-FISH) techniques in the detection 
      of genetic changes in chronic myeloid leukemia (CML) with variant Philadelphia 
      translocation (vPh). METHODS: Cytogenetic preparations from 10 CML patients with 
      vPh confirmed by R banding were assayed with dual color dual fusion FISH 
      technique. If only one fusion signal was detected in interphase cells, metaphase 
      cells were observed to determine if there were derivative chromosome 9[der (9)] 
      deletions. Meanwhile, the same cytogenetic preparations were assayed with M-FISH 
      technique. RESULTS: Of the 10 CML patients with vPh, 5 were detected with der (9) 
      deletions by FISH technique. M-FISH technique revealed that besides the 
      chromosome 22, chromosomes 1, 3, 5, 6, 8, 10, 11 and 17 were also involved in the 
      vPh. M-FISH technique also detected the abnormalities which were not found with 
      conventional cytogenetics (CC), including two never reported abnormalities. 
      CONCLUSION: The combination of CC, FISH and M-FISH technique could refine the 
      genetic diagnosis of CML with vPh.
FAU - Wu, Wei
AU  - Wu W
AD  - Department of Hematology, the First Affiliated Hospital of Nanjing Medical 
      University, Nanjing, Jiangsu, 210029 P. R. China.
FAU - Li, Jian-yong
AU  - Li JY
FAU - Zhu, Yu
AU  - Zhu Y
FAU - Qiu, Hai-rong
AU  - Qiu HR
FAU - Pan, Jin-lan
AU  - Pan JL
FAU - Xu, Wei
AU  - Xu W
FAU - Chen, Li-juan
AU  - Chen LJ
FAU - Shen, Yun-feng
AU  - Shen YF
FAU - Xue, Yong-quan
AU  - Xue YQ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - Adult
MH  - Aged
MH  - Chromosomes, Human, Pair 1/genetics
MH  - Chromosomes, Human, Pair 10/genetics
MH  - Chromosomes, Human, Pair 11/genetics
MH  - Chromosomes, Human, Pair 17/genetics
MH  - Chromosomes, Human, Pair 22/genetics
MH  - Chromosomes, Human, Pair 3/genetics
MH  - Chromosomes, Human, Pair 5/genetics
MH  - Chromosomes, Human, Pair 6/genetics
MH  - Chromosomes, Human, Pair 8/genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*diagnosis/*genetics
MH  - Male
MH  - Middle Aged
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Translocation, Genetic/genetics
MH  - Young Adult
EDAT- 2007/08/08 09:00
MHDA- 2009/03/28 09:00
CRDT- 2007/08/08 09:00
PHST- 2007/08/08 09:00 [pubmed]
PHST- 2009/03/28 09:00 [medline]
PHST- 2007/08/08 09:00 [entrez]
AID - 940624108 [pii]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2007 Aug;24(4):470-3.