PMID- 17681288
OWN - NLM
STAT- MEDLINE
DCOM- 20080102
LR  - 20071015
IS  - 0009-3084 (Print)
IS  - 0009-3084 (Linking)
VI  - 150
IP  - 2
DP  - 2007 Dec
TI  - The lipid composition of the unicellular green alga Chlamydomonas reinhardtii and 
      the diatom Cyclotella meneghiniana investigated by MALDI-TOF MS and TLC.
PG  - 143-55
AB  - The lipid composition of algae is crucial for numerous structural and 
      physiological aspects, e.g. the integrity of the photosynthetic complexes and the 
      functionality of membrane-embedded processes as the photosynthetic electron 
      transport in thylakoids or the mitochondrial respiration. In this paper the lipid 
      composition of the organic extracts of the green alga Chlamydomonas reinhardtii 
      and the diatom Cyclotella meneghiniana are compared by using matrix-assisted 
      laser desorption and ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) 
      in combination with thin-layer chromatography (TLC). The combined methods enable 
      quantitative evaluation of the individual lipid classes as well as the 
      determination of the relative acyl compositions. It will be shown that both algae 
      differ in (a) the lipid classes, (b) the relative contribution of the individual 
      lipid classes and (c) the acyl compositions. Differences in the acyl composition 
      concern particularly the mono- and digalactosyl diacylglycerols. 
      Glycerol-trimethylhomoserine and phosphatidylethanolamine are exclusively 
      detected in the C. reinhardtii extracts, whereas phosphatidylcholine is a 
      characteristic lipid of C. meneghiniana. Furthermore, the proportion of the 
      acidic lipids sulfoquinovosyl-diacylglycerol and phosphatidylglycerol is 
      significantly higher in the diatom than in C. reinhardtii.
FAU - Vieler, Astrid
AU  - Vieler A
AD  - University of Leipzig, Faculty of Biosciences, Pharmacy and Psychology, Institute 
      of Biology I, Johannisallee 21-23, D-04103 Leipzig, Germany.
FAU - Wilhelm, Christian
AU  - Wilhelm C
FAU - Goss, Reimund
AU  - Goss R
FAU - Suss, Rosmarie
AU  - Suss R
FAU - Schiller, Jurgen
AU  - Schiller J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070703
PL  - Ireland
TA  - Chem Phys Lipids
JT  - Chemistry and physics of lipids
JID - 0067206
RN  - 0 (Ions)
RN  - 0 (Lipids)
RN  - 1406-65-1 (Chlorophyll)
RN  - 36-88-4 (Carotenoids)
SB  - IM
MH  - Animals
MH  - Biochemistry/*methods
MH  - Carotenoids/chemistry
MH  - Chlamydomonas reinhardtii/*metabolism
MH  - Chlorophyll/chemistry
MH  - Chromatography, Thin Layer/*methods
MH  - Diatoms/*metabolism
MH  - Ions
MH  - Lipids/*chemistry
MH  - Models, Chemical
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
EDAT- 2007/08/08 09:00
MHDA- 2008/01/03 09:00
CRDT- 2007/08/08 09:00
PHST- 2007/02/27 00:00 [received]
PHST- 2007/06/26 00:00 [revised]
PHST- 2007/06/28 00:00 [accepted]
PHST- 2007/08/08 09:00 [pubmed]
PHST- 2008/01/03 09:00 [medline]
PHST- 2007/08/08 09:00 [entrez]
AID - S0009-3084(07)00386-6 [pii]
AID - 10.1016/j.chemphyslip.2007.06.224 [doi]
PST - ppublish
SO  - Chem Phys Lipids. 2007 Dec;150(2):143-55. doi: 10.1016/j.chemphyslip.2007.06.224. 
      Epub 2007 Jul 3.