PMID- 17681875
OWN - NLM
STAT- MEDLINE
DCOM- 20080918
LR  - 20220311
IS  - 1368-8375 (Print)
IS  - 1368-8375 (Linking)
VI  - 44
IP  - 4
DP  - 2008 Apr
TI  - Chromosomal imbalances in oral squamous cell carcinoma: examination of 31 cell 
      lines and review of the literature.
PG  - 369-82
AB  - Classical and molecular cytogenetic analysis, including fluorescence in situ 
      hybridization (FISH) and chromosomal comparative genomic hybridization (CGH), 
      were used to examine genetic changes involved in the development and/or 
      progression of oral squamous cell carcinoma (OSCC). Of 31 OSCC cell lines 
      studied, more than one-third expressed clonal structural abnormalities involving 
      chromosomes 3, 7, 8, 9, and 11. Eleven OSCC cell lines were evaluated using CGH 
      to identify novel genome-wide gains, losses, or amplifications. By CGH, more than 
      half of the cell lines showed loss of 3p, gain of 3q, 8q, and 20q. Further, 
      molecular cytogenetic analyses by FISH of primary tumors showed that the 
      karyotypes of cell lines derived from those tumors correlated with specific gains 
      and losses in the tumors from which they were derived. The most frequent 
      nonrandom aberration identified by both karyotype and CGH analyses was 
      amplification of chromosomal band 11q13 in the form of a homogeneously staining 
      region. Our data suggest that loss of 9p and 11q13 amplification may be of 
      prognostic benefit in the management of OSCC, which is consistent with the 
      literature. The results of this study validate the relationship between these 
      OSCC cell lines and the tumors from which they were derived. The results also 
      emphasize the usefulness of these cell lines as in vitro experimental models and 
      provide important genetic information on these OSCC cell lines that were recently 
      reported in this journal.
FAU - Martin, Christa Lese
AU  - Martin CL
AD  - Department of Human Genetics, University of Pittsburgh, Graduate School of Public 
      Health, 130 DeSoto Street, Pittsburgh, PA 15261, United States.
FAU - Reshmi, Shalini C
AU  - Reshmi SC
FAU - Ried, Thomas
AU  - Ried T
FAU - Gottberg, William
AU  - Gottberg W
FAU - Wilson, John W
AU  - Wilson JW
FAU - Reddy, Jaya K
AU  - Reddy JK
FAU - Khanna, Poornima
AU  - Khanna P
FAU - Johnson, Jonas T
AU  - Johnson JT
FAU - Myers, Eugene N
AU  - Myers EN
FAU - Gollin, Susanne M
AU  - Gollin SM
LA  - eng
GR  - P30 CA047904-119019/CA/NCI NIH HHS/United States
GR  - UL1 TR000005/TR/NCATS NIH HHS/United States
GR  - R01 DE014729-02/DE/NIDCR NIH HHS/United States
GR  - R01 DE016086-02/DE/NIDCR NIH HHS/United States
GR  - R01 DE016086/DE/NIDCR NIH HHS/United States
GR  - P30 CA047904-169019/CA/NCI NIH HHS/United States
GR  - P30 CA047904-11S29019/CA/NCI NIH HHS/United States
GR  - P30 CA047904-12S19019/CA/NCI NIH HHS/United States
GR  - P30 CA047904-14S19019/CA/NCI NIH HHS/United States
GR  - R01 DE016086-04/DE/NIDCR NIH HHS/United States
GR  - R01DE14729/DE/NIDCR NIH HHS/United States
GR  - P30 CA047904-149019/CA/NCI NIH HHS/United States
GR  - P50 CA097190-02/CA/NCI NIH HHS/United States
GR  - P50 CA097190-01A1/CA/NCI NIH HHS/United States
GR  - R01 DE014729-04/DE/NIDCR NIH HHS/United States
GR  - P30 CA047904/CA/NCI NIH HHS/United States
GR  - P30 CA047904-139019/CA/NCI NIH HHS/United States
GR  - P30 CA047904-11S39019/CA/NCI NIH HHS/United States
GR  - R01DE10513/DE/NIDCR NIH HHS/United States
GR  - P30 CA047904-109019/CA/NCI NIH HHS/United States
GR  - R01 DE014729-01/DE/NIDCR NIH HHS/United States
GR  - P60 DE013059/DE/NIDCR NIH HHS/United States
GR  - R01 DE014729/DE/NIDCR NIH HHS/United States
GR  - P50 CA097190-04/CA/NCI NIH HHS/United States
GR  - R01 DE010513-03/DE/NIDCR NIH HHS/United States
GR  - P60DE13059/DE/NIDCR NIH HHS/United States
GR  - P50 CA097190/CA/NCI NIH HHS/United States
GR  - P30CA47904/CA/NCI NIH HHS/United States
GR  - P60 DE013059-010004/DE/NIDCR NIH HHS/United States
GR  - P60 DE013059-030004/DE/NIDCR NIH HHS/United States
GR  - R01 DE014729-01S1/DE/NIDCR NIH HHS/United States
GR  - R01 DE014729-03/DE/NIDCR NIH HHS/United States
GR  - P60 DE013059-040004/DE/NIDCR NIH HHS/United States
GR  - R01 DE016086-01/DE/NIDCR NIH HHS/United States
GR  - P30 CA047904-159019/CA/NCI NIH HHS/United States
GR  - R01DE016086/DE/NIDCR NIH HHS/United States
GR  - P50 CA097190-03/CA/NCI NIH HHS/United States
GR  - R01 DE016086-03/DE/NIDCR NIH HHS/United States
GR  - P30 CA047904-12S29019/CA/NCI NIH HHS/United States
GR  - P30 CA047904-129019/CA/NCI NIH HHS/United States
GR  - P60 DE013059-020004/DE/NIDCR NIH HHS/United States
GR  - R01 DE014729-05/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20070802
PL  - England
TA  - Oral Oncol
JT  - Oral oncology
JID - 9709118
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 11/genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Mouth Neoplasms/*genetics/pathology
MH  - Neoplasm Staging
MH  - Nucleic Acid Hybridization
MH  - Prognosis
MH  - Tumor Cells, Cultured
PMC - PMC2362065
MID - NIHMS45931
COIS- Conflict of interest None of the authors have any financial or personal 
      relationships with other people or organizations that could inappropriately 
      influence or bias their contribution to this paper.
EDAT- 2007/08/08 09:00
MHDA- 2008/09/19 09:00
PMCR- 2009/04/01
CRDT- 2007/08/08 09:00
PHST- 2007/04/09 00:00 [received]
PHST- 2007/05/01 00:00 [revised]
PHST- 2007/05/02 00:00 [accepted]
PHST- 2007/08/08 09:00 [pubmed]
PHST- 2008/09/19 09:00 [medline]
PHST- 2007/08/08 09:00 [entrez]
PHST- 2009/04/01 00:00 [pmc-release]
AID - S1368-8375(07)00123-6 [pii]
AID - 10.1016/j.oraloncology.2007.05.003 [doi]
PST - ppublish
SO  - Oral Oncol. 2008 Apr;44(4):369-82. doi: 10.1016/j.oraloncology.2007.05.003. Epub 
      2007 Aug 2.