PMID- 17683740
OWN - NLM
STAT- MEDLINE
DCOM- 20070831
LR  - 20131121
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 597
IP  - 2
DP  - 2007 Aug 6
TI  - Quantitative analysis of clavulanic acid in porcine tissues by liquid 
      chromatography combined with electrospray ionization tandem mass spectrometry.
PG  - 282-9
AB  - The purpose of this study was to develop and validate a method for the 
      determination of clavulanic acid (CLAV) residues in edible tissues of swine by 
      liquid chromatography-electrospray ionisation-tandem mass spectrometry 
      (LC-ESI-MS/MS). After a simple extraction of CLAV using an aqueous phosphate 
      buffer solution of pH 6.0, an ultrafiltration step was performed for protein 
      removal. Chromatography of CLAV and the internal standard tazobactam (TAZO) was 
      achieved on a reversed-phase PLRP-S polymeric column (150 mm x 2.1 mm i.d., 100 
      A) using a mixture of 0.05 (v/v)% formic acid in water and acetonitrile. The mass 
      spectrometer was operated in the MS/MS selected reaction monitoring (SRM) mode. 
      The method was validated for the analysis of porcine muscle, skin plus fat, liver 
      and kidney, according to the requirements defined by the European Community. 
      Calibration curves were prepared for all tissues and good linearity was achieved 
      over the concentration ranges tested (correlation coefficient > or = 0.99 and 
      goodness-of-fit coefficient < or = 10%). Limits of quantification of 50 ng g(-1) 
      were obtained for the analysis of CLAV in the various tissues which corresponds 
      in all cases to at least half the maximum residue limits (MRLs). Limits of 
      detection ranged between 8.0 and 15.14 ng g(-1). The within-day, between-day 
      precisions and trueness fell within the ranges specified in the 
      EMEA/CVMP/573-00/FINAL document. Biological samples from pigs that received an 
      oral or intravenous bolus of a commercial amoxicillin/clavulanic acid formulation 
      were analyzed using the described method.
FAU - Reyns, Tim
AU  - Reyns T
AD  - Ghent University, Faculty of Veterinary Medicine, Department of Pharmacology, 
      Toxicology, Biochemistry and Organ Physiology, Salisburylaan 133, B-9820 
      Merelbeke, Belgium. Tim.Reyns@UGent.be
FAU - De Boever, Sandra
AU  - De Boever S
FAU - De Baere, Siegrid
AU  - De Baere S
FAU - De Backer, Patrick
AU  - De Backer P
FAU - Croubels, Siska
AU  - Croubels S
LA  - eng
PT  - Journal Article
DEP - 20070705
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 23521W1S24 (Clavulanic Acid)
SB  - IM
MH  - Animals
MH  - Chromatography, Liquid/*methods
MH  - Clavulanic Acid/*analysis/chemistry
MH  - Molecular Structure
MH  - Organ Specificity
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Swine
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2007/08/09 09:00
MHDA- 2007/09/01 09:00
CRDT- 2007/08/09 09:00
PHST- 2007/05/21 00:00 [received]
PHST- 2007/06/29 00:00 [revised]
PHST- 2007/06/30 00:00 [accepted]
PHST- 2007/08/09 09:00 [pubmed]
PHST- 2007/09/01 09:00 [medline]
PHST- 2007/08/09 09:00 [entrez]
AID - S0003-2670(07)01155-5 [pii]
AID - 10.1016/j.aca.2007.06.056 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2007 Aug 6;597(2):282-9. doi: 10.1016/j.aca.2007.06.056. Epub 
      2007 Jul 5.