PMID- 17684537
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20170519
IS  - 1673-7067 (Print)
IS  - 1995-8218 (Linking)
VI  - 22
IP  - 1
DP  - 2006 Jan
TI  - Repair of spinal cord injury by neural stem cells modified with BDNF gene in 
      rats.
PG  - 34-40
AB  - Objective To explore repair of spinal cord injury by neural stem cells (NSCs) 
      modified with brain derived neurotrophic factor (BDNF) gene (BDNF-NSCs) in rats. 
      Methods Neural stem cells modified with BDNF gene were transplanted into the 
      complete transection site of spinal cord at the lumbar 4 (L4) level in rats. 
      Motor function of rats' hind limbs was observed and HE and X-gal 
      immunocytochemical staining, in situ hybridization, and retrograde HRP tracing 
      were also performed. Results BDNF-NSCs survived and integrated well with host 
      spinal cord. In the transplant group, some X-gal positive, NF-200 positive, GFAP 
      positive, BDNF positive, and BDNF mRNA positive cells, and many NF-200 positive 
      nerve fibers were observed in the injury site. Retrograde HRP tracing through 
      sciatic nerve showed some HRP positive cells and nerve fibers near the rostral 
      side of the injury one month after transplant and with time, they increased in 
      number. Examinations on rats' motor function and behavior demonstrated that motor 
      function of rats' hind limbs improved better in the transplant group than the 
      injury group. Conclusion BDNF-NSCs can survive, differentiate, and partially 
      integrate with host spinal cord, and they significantly ameliorate rats ' motor 
      function of hind limbs, indicating their promising role in repairing spinal cord 
      injury.
FAU - Li, Wei
AU  - Li W
AD  - School of Nursing; Department of Histology and Embryology, Third Military Medical 
      University, Chongqing 400038, China E-mail: weilee2004@yahoo.com.cn.
FAU - Cai, Wen-Qin
AU  - Cai WQ
FAU - Li, Cheng-Ren
AU  - Li CR
LA  - eng
PT  - Journal Article
PL  - Singapore
TA  - Neurosci Bull
JT  - Neuroscience bulletin
JID - 101256850
EDAT- 2007/08/09 09:00
MHDA- 2007/08/09 09:01
CRDT- 2007/08/09 09:00
PHST- 2007/08/09 09:00 [pubmed]
PHST- 2007/08/09 09:01 [medline]
PHST- 2007/08/09 09:00 [entrez]
PST - ppublish
SO  - Neurosci Bull. 2006 Jan;22(1):34-40.