PMID- 17686020
OWN - NLM
STAT- MEDLINE
DCOM- 20071016
LR  - 20070809
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 9
IP  - 9
DP  - 2007 Sep
TI  - Effects of environmental conditions on the nitrifying population dynamics in a 
      pilot wastewater treatment plant.
PG  - 2220-33
AB  - The effect of environmental conditions, especially ammonium concentration, on 
      community composition and nitrification activity of nitrifying bacterial biofilms 
      in a pilot wastewater treatment plant was examined. A decreasing ammonium 
      gradient was created when four aerated tanks with suspended carrier material were 
      serially fed with wastewater. Community composition was analysed using 
      fluorescence in situ hybridization (FISH) probes as well as partial 16S rRNA and 
      amoA gene analysis using polymerase chain reaction-denaturating gradient gel 
      electrophoresis (PCR-DGGE) and sequencing. Fluorescence in situ hybridization 
      probes identified at least five ammonia-oxidizing bacterial (AOB) and two 
      nitrite-oxidizing bacterial (NOB) populations. A change in nitrifying community 
      was detected in the tanks, indicating that ammonium was an important structuring 
      factor. Further, we found support for different autoecology within the 
      Nitrosomonas oligotropha lineage, as at least one population within this lineage 
      increased in relative abundance with ammonium concentration while another 
      population decreased. Absolute numbers of AOB and NOB growing in biofilms on the 
      carriers were determined and the cell specific nitrification rates calculated 
      seemed strongly correlated to ammonium concentration. Oxygen could also be 
      limiting in the biofilms of the first tank with high ammonium concentrations. The 
      response of the nitrifying community to increased ammonium concentrations 
      differed between the tanks, indicating that activity correlates with community 
      structure.
FAU - Lydmark, Par
AU  - Lydmark P
AD  - Department of Cell and Molecular Biology, Microbiology, Goteborg University, 
      Goteborg, Sweden.
FAU - Almstrand, Robert
AU  - Almstrand R
FAU - Samuelsson, Kristina
AU  - Samuelsson K
FAU - Mattsson, Ann
AU  - Mattsson A
FAU - Sorensson, Fred
AU  - Sorensson F
FAU - Lindgren, Per-Eric
AU  - Lindgren PE
FAU - Hermansson, Malte
AU  - Hermansson M
LA  - eng
SI  - GENBANK/EF530682
SI  - GENBANK/EF530683
SI  - GENBANK/EF530684
SI  - GENBANK/EF530685
SI  - GENBANK/EF530686
SI  - GENBANK/EF530687
SI  - GENBANK/EF530688
SI  - GENBANK/EF530689
SI  - GENBANK/EF530690
SI  - GENBANK/EF530691
SI  - GENBANK/EF530692
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (Nitrites)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
SB  - IM
MH  - Bacteria/genetics/*growth & development/metabolism
MH  - Biofilms
MH  - Ecosystem
MH  - Molecular Sequence Data
MH  - Nitrites/*metabolism
MH  - Phylogeny
MH  - Pilot Projects
MH  - Quaternary Ammonium Compounds/*metabolism
MH  - RNA, Ribosomal, 16S/classification
MH  - Sewage/*microbiology
MH  - *Waste Disposal, Fluid
EDAT- 2007/08/10 09:00
MHDA- 2007/10/17 09:00
CRDT- 2007/08/10 09:00
PHST- 2007/08/10 09:00 [pubmed]
PHST- 2007/10/17 09:00 [medline]
PHST- 2007/08/10 09:00 [entrez]
AID - EMI1336 [pii]
AID - 10.1111/j.1462-2920.2007.01336.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2007 Sep;9(9):2220-33. doi: 10.1111/j.1462-2920.2007.01336.x.