PMID- 17686876
OWN - NLM
STAT- MEDLINE
DCOM- 20080108
LR  - 20181113
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 81
IP  - 20
DP  - 2007 Oct
TI  - Characterization of human immunodeficiency virus type 1 replication in immature 
      and mature dendritic cells reveals dissociable cis- and trans-infection.
PG  - 11352-62
AB  - Dendritic cells (DCs) transmit human immunodeficiency virus type 1 (HIV-1) to 
      CD4(+) T cells through the trans- and cis-infection pathways; however, little is 
      known about the relative efficiencies of these pathways and whether they are 
      interdependent. Here we compare cis- and trans-infections of HIV-1 mediated by 
      immature DCs (iDCs) and mature DCs (mDCs), using replication-competent and 
      single-cycle HIV-1. Monocyte-derived iDCs were differentiated into various types 
      of mDCs by lipopolysaccharide (LPS), tumor necrosis factor alpha (TNF-alpha), and 
      CD40 ligand (CD40L). iDCs and CD40L-induced mDCs were susceptible to HIV-1 
      infection and mediated efficient viral transmission to CD4(+) T cells. Although 
      HIV-1 cis-infection was partially restricted in TNF-alpha-induced mDCs and 
      profoundly blocked in LPS-induced mDCs, these cells efficiently promoted HIV-1 
      trans-infection of CD4(+) T cells. The postentry restriction of HIV-1 infection 
      in LPS-induced mDCs was identified at the levels of reverse transcription and 
      postintegration, using real-time PCR quantification of viral DNA and integration. 
      Furthermore, nucleofection of DCs with HIV-1 proviral DNA confirmed that impaired 
      gene expression of LPS-induced mDCs was responsible for the postentry restriction 
      of HIV-1 infection. Our results suggest that various DC subsets in vivo may 
      differentially contribute to HIV-1 dissemination via dissociable cis- and 
      trans-infections.
FAU - Dong, Chunsheng
AU  - Dong C
AD  - Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 
      8701 Watertown Plank Road, Milwaukee, WI 53226, USA.
FAU - Janas, Alicia M
AU  - Janas AM
FAU - Wang, Jian-Hua
AU  - Wang JH
FAU - Olson, Wendy J
AU  - Olson WJ
FAU - Wu, Li
AU  - Wu L
LA  - eng
GR  - R01 AI068493/AI/NIAID NIH HHS/United States
GR  - R01 AI068493-02/AI/NIAID NIH HHS/United States
GR  - R01-AI068493/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20070808
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA, Viral)
SB  - IM
MH  - CD4-Positive T-Lymphocytes/*virology
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - DNA, Viral
MH  - Dendritic Cells/pathology/*virology
MH  - HIV Infections
MH  - HIV-1/*pathogenicity
MH  - Humans
MH  - Virus Integration
MH  - *Virus Replication
PMC - PMC2045571
EDAT- 2007/08/10 09:00
MHDA- 2008/01/09 09:00
PMCR- 2008/02/01
CRDT- 2007/08/10 09:00
PHST- 2007/08/10 09:00 [pubmed]
PHST- 2008/01/09 09:00 [medline]
PHST- 2007/08/10 09:00 [entrez]
PHST- 2008/02/01 00:00 [pmc-release]
AID - JVI.01081-07 [pii]
AID - 1081-07 [pii]
AID - 10.1128/JVI.01081-07 [doi]
PST - ppublish
SO  - J Virol. 2007 Oct;81(20):11352-62. doi: 10.1128/JVI.01081-07. Epub 2007 Aug 8.