PMID- 17688372
OWN - NLM
STAT- MEDLINE
DCOM- 20071024
LR  - 20070810
IS  - 0028-2685 (Print)
IS  - 0028-2685 (Linking)
VI  - 54
IP  - 5
DP  - 2007
TI  - Establishing the method of chimerism monitoring after allogeneic stem cell 
      transplantation using multiplex polymerase chain reaction amplification of short 
      tandem repeat markers and Amelogenin.
PG  - 424-30
AB  - We describe the implementation, optimization, sensitivity determination and first 
      clinical results of polymerase chain reaction (PCR) amplification of polymorphic 
      short tandem repeat (STR) markers and Amelogenin locus coupled with fluorescent 
      detection and capillary electrophoresis in chimerism monitoring of patients 
      transplanted at three different transplant centers using a commercially available 
      multiplex microsatellite assay. The chimerism analysis was performed with genomic 
      DNA extracted from unselected peripheral blood leukocytes of one hundred 
      pediatric and adult patients, who underwent allogeneic stem cell transplantation 
      (SCT) from human leukocyte antigen (HLA) matched or one antigen mismatched 
      related or unrelated donors for malignant (70 patients) and non-malignant (30 
      patients) diseases. Tested were 79 donor recipient pairs for 15 STR systems and 
      identified an informative marker in all but one of them (98,7%), using 6 selected 
      systems out of these fifteen, that appeared highly informative in our patients 
      population. In 21 sex-mismatched donor recipient pairs we used the Amelogenin 
      locus to distinguish the X and Y chromosome. In sixty-three out of these 100 
      patients chimerism was regularly analyzed from blood samples taken at various 
      time points after SCT with the median follow up of 17 months. Complete chimerism 
      (CC), maintained over the whole follow-up period, was detected in 24 (38, 1%), 
      stable and decreasing mixed chimerism (MC) in 28 (44, 4%) and increasing MC in 11 
      patients (17, 5%). Patients with CC, stable and decreasing MC showed a 
      significantly better (p 0,005) overall survival rate (0, 81), compared to those 
      with increasing MC (0, 24). These results demonstrate that STR-based chimerism 
      monitoring with sensitivity above 1% and high informativity (98, 7% of donor 
      recipient pairs) is necessary in establishing the origin of engrafted cells after 
      an allogeneic SCT, in detecting graft rejection and that it may contribute in 
      identifying patients with imminent leukemia relapse.
FAU - Sufliarska, S
AU  - Sufliarska S
AD  - Bone Marrow Transplantation Unit, Department of Pediatrics, Comenius University 
      Medical School, Limbova 1, 833 40 Bratislava, Slovak Republic. 
      sufliarska@dfnsp.sk
FAU - Minarik, G
AU  - Minarik G
FAU - Horakova, J
AU  - Horakova J
FAU - Bodova, I
AU  - Bodova I
FAU - Bojtarova, E
AU  - Bojtarova E
FAU - Czako, B
AU  - Czako B
FAU - Mistrik, M
AU  - Mistrik M
FAU - Drgona, L
AU  - Drgona L
FAU - Demitrovicova, M
AU  - Demitrovicova M
FAU - Lakota, J
AU  - Lakota J
FAU - Krivosikova, M
AU  - Krivosikova M
FAU - Kovacs, L
AU  - Kovacs L
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
PL  - Slovakia
TA  - Neoplasma
JT  - Neoplasma
JID - 0377266
RN  - 0 (Amelogenin)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Amelogenin/*genetics
MH  - Child
MH  - Child, Preschool
MH  - Chromosome Mapping
MH  - Female
MH  - *Gene Amplification
MH  - Genetic Markers
MH  - Humans
MH  - Infant
MH  - Leukemia/genetics/mortality/*therapy
MH  - Lymphoma/genetics/mortality/therapy
MH  - Male
MH  - Middle Aged
MH  - Monitoring, Physiologic
MH  - *Polymerase Chain Reaction
MH  - Recurrence
MH  - *Stem Cell Transplantation
MH  - Survival Analysis
MH  - Tandem Repeat Sequences
MH  - *Transplantation Chimera
MH  - Transplantation, Homologous
EDAT- 2007/08/11 09:00
MHDA- 2007/10/25 09:00
CRDT- 2007/08/11 09:00
PHST- 2007/08/11 09:00 [pubmed]
PHST- 2007/10/25 09:00 [medline]
PHST- 2007/08/11 09:00 [entrez]
PST - ppublish
SO  - Neoplasma. 2007;54(5):424-30.